1zt5
From Proteopedia
(New page: 200px<br /> <applet load="1zt5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zt5, resolution 1.818Å" /> '''C-terminal domain ...) |
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| - | [[Image:1zt5.gif|left|200px]]<br /> | + | [[Image:1zt5.gif|left|200px]]<br /><applet load="1zt5" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1zt5" size=" | + | |
caption="1zt5, resolution 1.818Å" /> | caption="1zt5, resolution 1.818Å" /> | ||
'''C-terminal domain of Insulin-like Growth Factor Binding Protein-1 isolated from human amniotic fluid complexed with Iron(II)'''<br /> | '''C-terminal domain of Insulin-like Growth Factor Binding Protein-1 isolated from human amniotic fluid complexed with Iron(II)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Insulin-like growth factor (IGF)-binding protein-1 (IGFBP-1) regulates the | + | Insulin-like growth factor (IGF)-binding protein-1 (IGFBP-1) regulates the activity of the insulin-like growth factors in early pregnancy and is, thus, thought to play a key role at the fetal-maternal interface. The C-terminal domain of IGFBP-1 and three isoforms of the intact protein were isolated from human amniotic fluid, and sequencing of the four N-terminal polypeptide chains showed them to be highly pure. The addition of both intact IGFBP-1 and its C-terminal fragment to cultured fibroblasts has a similar stimulating effect on cell migration, and therefore, the domain has a biological activity on its own. The three-dimensional structure of the C-terminal domain was determined by x-ray crystallography to 1.8 Angstroms resolution. The fragment folds as a thyroglobulin type I domain and was found to bind the Fe(2+) ion in the crystals through the only histidine residue present in the polypeptide chain. Iron (II) decreases the binding of intact IGFBP-1 and the C-terminal domain to IGF-II, suggesting that the metal binding site is close to or part of the surface of interaction of the two molecules. |
==About this Structure== | ==About this Structure== | ||
| - | 1ZT5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with FE2 and DIO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1ZT5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=FE2:'>FE2</scene> and <scene name='pdbligand=DIO:'>DIO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZT5 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Campagnoli, M.]] | [[Category: Campagnoli, M.]] | ||
[[Category: Capaldi, S.]] | [[Category: Capaldi, S.]] | ||
| - | [[Category: Carrizo, M | + | [[Category: Carrizo, M E.]] |
[[Category: Faggion, B.]] | [[Category: Faggion, B.]] | ||
[[Category: Galliano, M.]] | [[Category: Galliano, M.]] | ||
[[Category: Labo, S.]] | [[Category: Labo, S.]] | ||
[[Category: Minchiotti, L.]] | [[Category: Minchiotti, L.]] | ||
| - | [[Category: Monaco, H | + | [[Category: Monaco, H L.]] |
[[Category: Perduca, M.]] | [[Category: Perduca, M.]] | ||
[[Category: Romano, A.]] | [[Category: Romano, A.]] | ||
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[[Category: metal-binding]] | [[Category: metal-binding]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:18:49 2008'' |
Revision as of 14:18, 21 February 2008
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C-terminal domain of Insulin-like Growth Factor Binding Protein-1 isolated from human amniotic fluid complexed with Iron(II)
Overview
Insulin-like growth factor (IGF)-binding protein-1 (IGFBP-1) regulates the activity of the insulin-like growth factors in early pregnancy and is, thus, thought to play a key role at the fetal-maternal interface. The C-terminal domain of IGFBP-1 and three isoforms of the intact protein were isolated from human amniotic fluid, and sequencing of the four N-terminal polypeptide chains showed them to be highly pure. The addition of both intact IGFBP-1 and its C-terminal fragment to cultured fibroblasts has a similar stimulating effect on cell migration, and therefore, the domain has a biological activity on its own. The three-dimensional structure of the C-terminal domain was determined by x-ray crystallography to 1.8 Angstroms resolution. The fragment folds as a thyroglobulin type I domain and was found to bind the Fe(2+) ion in the crystals through the only histidine residue present in the polypeptide chain. Iron (II) decreases the binding of intact IGFBP-1 and the C-terminal domain to IGF-II, suggesting that the metal binding site is close to or part of the surface of interaction of the two molecules.
About this Structure
1ZT5 is a Single protein structure of sequence from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
Reference
Structure and properties of the C-terminal domain of insulin-like growth factor-binding protein-1 isolated from human amniotic fluid., Sala A, Capaldi S, Campagnoli M, Faggion B, Labo S, Perduca M, Romano A, Carrizo ME, Valli M, Visai L, Minchiotti L, Galliano M, Monaco HL, J Biol Chem. 2005 Aug 19;280(33):29812-9. Epub 2005 Jun 22. PMID:15972819
Page seeded by OCA on Thu Feb 21 16:18:49 2008
Categories: Homo sapiens | Single protein | Campagnoli, M. | Capaldi, S. | Carrizo, M E. | Faggion, B. | Galliano, M. | Labo, S. | Minchiotti, L. | Monaco, H L. | Perduca, M. | Romano, A. | Sala, A. | Valli, M. | Visai, L. | DIO | FE2 | Amniotic fluid | C-terminal domain | Igfbp-1 | Insulin-like growth factor binding protein-1 | Iron | Metal-binding
