1zta
From Proteopedia
(New page: 200px<br /><applet load="1zta" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zta" /> '''THE SOLUTION STRUCTURE OF A LEUCINE-ZIPPER M...) |
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| - | [[Image:1zta.gif|left|200px]]<br /><applet load="1zta" size=" | + | [[Image:1zta.gif|left|200px]]<br /><applet load="1zta" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''THE SOLUTION STRUCTURE OF A LEUCINE-ZIPPER MOTIF PEPTIDE'''<br /> | '''THE SOLUTION STRUCTURE OF A LEUCINE-ZIPPER MOTIF PEPTIDE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We report the complete structure determination of a 34 residue synthetic | + | We report the complete structure determination of a 34 residue synthetic peptide with the amino acid sequence of the dimerization domain (leucine zipper) of GCN4. A high resolution structure in solution was obtained by 1H-NMR studies and distance geometry calculations followed by restrained energy minimization. A set of 20 final structures was obtained with an average root mean square deviation of 1.3 A for the backbone atoms (excluding the first and the last two residues). The structure contains an uninterrupted helix. A comparison with a structure previously determined for a larger peptide containing both the DNA-binding region (basic region) and the leucine-zipper motif shows the structural independence of the leucine-zipper domain from the contiguous DNA binding region. |
==About this Structure== | ==About this Structure== | ||
| - | 1ZTA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http:// | + | 1ZTA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZTA OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Gausepohl, H.]] | [[Category: Gausepohl, H.]] | ||
[[Category: Gibson, T.]] | [[Category: Gibson, T.]] | ||
| - | [[Category: Morelli, M | + | [[Category: Morelli, M A.Castiglione.]] |
[[Category: Pastore, A.]] | [[Category: Pastore, A.]] | ||
[[Category: Saudek, V.]] | [[Category: Saudek, V.]] | ||
[[Category: dna-binding motif]] | [[Category: dna-binding motif]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:18:50 2008'' |
Revision as of 14:18, 21 February 2008
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THE SOLUTION STRUCTURE OF A LEUCINE-ZIPPER MOTIF PEPTIDE
Overview
We report the complete structure determination of a 34 residue synthetic peptide with the amino acid sequence of the dimerization domain (leucine zipper) of GCN4. A high resolution structure in solution was obtained by 1H-NMR studies and distance geometry calculations followed by restrained energy minimization. A set of 20 final structures was obtained with an average root mean square deviation of 1.3 A for the backbone atoms (excluding the first and the last two residues). The structure contains an uninterrupted helix. A comparison with a structure previously determined for a larger peptide containing both the DNA-binding region (basic region) and the leucine-zipper motif shows the structural independence of the leucine-zipper domain from the contiguous DNA binding region.
About this Structure
1ZTA is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
The solution structure of a leucine-zipper motif peptide., Saudek V, Pastore A, Morelli MA, Frank R, Gausepohl H, Gibson T, Protein Eng. 1991 Jun;4(5):519-29. PMID:1891459
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