272d
From Proteopedia
(New page: 200px<br /><applet load="272d" size="350" color="white" frame="true" align="right" spinBox="true" caption="272d, resolution 2.000Å" /> '''PARALLEL AND ANTIPA...) |
|||
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | Nucleic acid triplexes are formed by sequence-specific interactions | + | Nucleic acid triplexes are formed by sequence-specific interactions between single-stranded polynucleotides and the double helix. These triplexes are implicated in genetic recombination in vivo and have application to areas that include genome analysis and antigene therapy. Despite the importance of the triple helix, only limited high-resolution structural information is available. The x-ray crystal structure of the oligonucleotide d(GGCCAATTGG) is described; it was designed to contain the d(G middle dotGC)2 fragment and thus provide the basic repeat unit of a DNA triple helix. Parameters derived from this crystal structure have made it possible to construct models of both parallel and antiparallel triple helices. |
==About this Structure== | ==About this Structure== | ||
| Line 15: | Line 15: | ||
[[Category: Gallois, B.]] | [[Category: Gallois, B.]] | ||
[[Category: Kennard, O.]] | [[Category: Kennard, O.]] | ||
| - | [[Category: Meervelt, L | + | [[Category: Meervelt, L Van.]] |
[[Category: Precigoux, G.]] | [[Category: Precigoux, G.]] | ||
[[Category: Vlieghe, D.]] | [[Category: Vlieghe, D.]] | ||
| Line 23: | Line 23: | ||
[[Category: overhanging bases]] | [[Category: overhanging bases]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:22:04 2008'' |
Revision as of 14:22, 21 February 2008
|
PARALLEL AND ANTIPARALLEL (G.GC)2 TRIPLE HELIX FRAGMENTS IN A CRYSTAL STRUCTURE
Overview
Nucleic acid triplexes are formed by sequence-specific interactions between single-stranded polynucleotides and the double helix. These triplexes are implicated in genetic recombination in vivo and have application to areas that include genome analysis and antigene therapy. Despite the importance of the triple helix, only limited high-resolution structural information is available. The x-ray crystal structure of the oligonucleotide d(GGCCAATTGG) is described; it was designed to contain the d(G middle dotGC)2 fragment and thus provide the basic repeat unit of a DNA triple helix. Parameters derived from this crystal structure have made it possible to construct models of both parallel and antiparallel triple helices.
About this Structure
272D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Parallel and antiparallel (G.GC)2 triple helix fragments in a crystal structure., Vlieghe D, Van Meervelt L, Dautant A, Gallois B, Precigoux G, Kennard O, Science. 1996 Sep 20;273(5282):1702-5. PMID:8781231
Page seeded by OCA on Thu Feb 21 16:22:04 2008
