This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
283d
From Proteopedia
(New page: 200px<br /><applet load="283d" size="350" color="white" frame="true" align="right" spinBox="true" caption="283d, resolution 2.300Å" /> '''A CURVED RNA HELIX ...) |
|||
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the RNA dodecamer 5'-GGCC(GAAA)GGCC-3' has been | + | The crystal structure of the RNA dodecamer 5'-GGCC(GAAA)GGCC-3' has been determined from x-ray diffraction data to 2.3-A resolution. In the crystal, these oligomers form double helices around twofold symmetry axes. Four consecutive non-Watson-Crick base pairs make up an internal loop in the middle of the duplex, including sheared G.A pairs and novel asymmetric A.A pairs. This internal loop sequence produces a significant curvature and narrowing of the double helix. The helix is curved by 34 degrees from end to end and the diameter is narrowed by 24% in the internal loop. A Mn2+ ion is bound directly to the N7 of the first guanine in the Watson-Crick region following the internal loop and the phosphate of the preceding residue. This Mn2+ location corresponds to a metal binding site observed in the hammerhead catalytic RNA. |
==About this Structure== | ==About this Structure== | ||
| Line 13: | Line 13: | ||
[[Category: ]] | [[Category: ]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Baeyens, K | + | [[Category: Baeyens, K J.]] |
| - | [[Category: Bondt, H | + | [[Category: Bondt, H L.De.]] |
| - | [[Category: Holbrook, S | + | [[Category: Holbrook, S R.]] |
[[Category: Pardi, A.]] | [[Category: Pardi, A.]] | ||
[[Category: MN]] | [[Category: MN]] | ||
| Line 24: | Line 24: | ||
[[Category: unusual rna]] | [[Category: unusual rna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:22:13 2008'' |
Revision as of 14:22, 21 February 2008
|
A CURVED RNA HELIX INCORPORATING AN INTERNAL LOOP WITH G-A AND A-A NON-WATSON-CRICK BASE PAIRING
Overview
The crystal structure of the RNA dodecamer 5'-GGCC(GAAA)GGCC-3' has been determined from x-ray diffraction data to 2.3-A resolution. In the crystal, these oligomers form double helices around twofold symmetry axes. Four consecutive non-Watson-Crick base pairs make up an internal loop in the middle of the duplex, including sheared G.A pairs and novel asymmetric A.A pairs. This internal loop sequence produces a significant curvature and narrowing of the double helix. The helix is curved by 34 degrees from end to end and the diameter is narrowed by 24% in the internal loop. A Mn2+ ion is bound directly to the N7 of the first guanine in the Watson-Crick region following the internal loop and the phosphate of the preceding residue. This Mn2+ location corresponds to a metal binding site observed in the hammerhead catalytic RNA.
About this Structure
283D is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
A curved RNA helix incorporating an internal loop with G.A and A.A non-Watson-Crick base pairing., Baeyens KJ, De Bondt HL, Pardi A, Holbrook SR, Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12851-5. PMID:8917508 [[Category: ]]
Page seeded by OCA on Thu Feb 21 16:22:13 2008
