283d
From Proteopedia
(New page: 200px<br /><applet load="283d" size="350" color="white" frame="true" align="right" spinBox="true" caption="283d, resolution 2.300Å" /> '''A CURVED RNA HELIX ...) |
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==Overview== | ==Overview== | ||
| - | The crystal structure of the RNA dodecamer 5'-GGCC(GAAA)GGCC-3' has been | + | The crystal structure of the RNA dodecamer 5'-GGCC(GAAA)GGCC-3' has been determined from x-ray diffraction data to 2.3-A resolution. In the crystal, these oligomers form double helices around twofold symmetry axes. Four consecutive non-Watson-Crick base pairs make up an internal loop in the middle of the duplex, including sheared G.A pairs and novel asymmetric A.A pairs. This internal loop sequence produces a significant curvature and narrowing of the double helix. The helix is curved by 34 degrees from end to end and the diameter is narrowed by 24% in the internal loop. A Mn2+ ion is bound directly to the N7 of the first guanine in the Watson-Crick region following the internal loop and the phosphate of the preceding residue. This Mn2+ location corresponds to a metal binding site observed in the hammerhead catalytic RNA. |
==About this Structure== | ==About this Structure== | ||
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[[Category: ]] | [[Category: ]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Baeyens, K | + | [[Category: Baeyens, K J.]] |
| - | [[Category: Bondt, H | + | [[Category: Bondt, H L.De.]] |
| - | [[Category: Holbrook, S | + | [[Category: Holbrook, S R.]] |
[[Category: Pardi, A.]] | [[Category: Pardi, A.]] | ||
[[Category: MN]] | [[Category: MN]] | ||
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[[Category: unusual rna]] | [[Category: unusual rna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:22:13 2008'' |
Revision as of 14:22, 21 February 2008
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A CURVED RNA HELIX INCORPORATING AN INTERNAL LOOP WITH G-A AND A-A NON-WATSON-CRICK BASE PAIRING
Overview
The crystal structure of the RNA dodecamer 5'-GGCC(GAAA)GGCC-3' has been determined from x-ray diffraction data to 2.3-A resolution. In the crystal, these oligomers form double helices around twofold symmetry axes. Four consecutive non-Watson-Crick base pairs make up an internal loop in the middle of the duplex, including sheared G.A pairs and novel asymmetric A.A pairs. This internal loop sequence produces a significant curvature and narrowing of the double helix. The helix is curved by 34 degrees from end to end and the diameter is narrowed by 24% in the internal loop. A Mn2+ ion is bound directly to the N7 of the first guanine in the Watson-Crick region following the internal loop and the phosphate of the preceding residue. This Mn2+ location corresponds to a metal binding site observed in the hammerhead catalytic RNA.
About this Structure
283D is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
A curved RNA helix incorporating an internal loop with G.A and A.A non-Watson-Crick base pairing., Baeyens KJ, De Bondt HL, Pardi A, Holbrook SR, Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12851-5. PMID:8917508 [[Category: ]]
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