296d
From Proteopedia
(New page: 200px<br /><applet load="296d" size="350" color="white" frame="true" align="right" spinBox="true" caption="296d, resolution 2.250Å" /> '''SEQUENCE-DEPENDENT ...) |
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==Overview== | ==Overview== | ||
| - | The bis-benzimidazole drug Hoechst 33258 has been co-crystallized with the | + | The bis-benzimidazole drug Hoechst 33258 has been co-crystallized with the dodecanucleotide sequence d(CGCAAATTTGCG)2. The structure has been solved by molecular replacement and refined to an R factor of 18.5% for 2125 reflections collected on a Xentronics area detector. The drug is bound in the minor groove, at the five base-pair site 5'-ATTTG and is in a unique orientation. This is displaced by one base pair in the 5' direction compared to previously-determined structures of this drug with the sequence d(CGCGAATTCGCG)2. Reasons for this difference in behaviour are discussed in terms of several sequence-dependent structural features of the DNA, with particular reference to differences in propeller twist and minor-groove width. |
==About this Structure== | ==About this Structure== | ||
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Sequence-dependent effects in drug-DNA interaction: the crystal structure of Hoechst 33258 bound to the d(CGCAAATTTGCG)2 duplex., Spink N, Brown DG, Skelly JV, Neidle S, Nucleic Acids Res. 1994 May 11;22(9):1607-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7515488 7515488] | Sequence-dependent effects in drug-DNA interaction: the crystal structure of Hoechst 33258 bound to the d(CGCAAATTTGCG)2 duplex., Spink N, Brown DG, Skelly JV, Neidle S, Nucleic Acids Res. 1994 May 11;22(9):1607-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7515488 7515488] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Brown, D | + | [[Category: Brown, D G.]] |
[[Category: Neidle, S.]] | [[Category: Neidle, S.]] | ||
| - | [[Category: Skelly, J | + | [[Category: Skelly, J V.]] |
[[Category: Spink, N.]] | [[Category: Spink, N.]] | ||
[[Category: HT]] | [[Category: HT]] | ||
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[[Category: double helix]] | [[Category: double helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:22:17 2008'' |
Revision as of 14:22, 21 February 2008
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SEQUENCE-DEPENDENT EFFECTS IN DRUG-DNA INTERACTION: THE CRYSTAL STRUCTURE OF HOECHST 33258 BOUND TO THE D(CGCAAATTTGCG)2 DUPLEX
Overview
The bis-benzimidazole drug Hoechst 33258 has been co-crystallized with the dodecanucleotide sequence d(CGCAAATTTGCG)2. The structure has been solved by molecular replacement and refined to an R factor of 18.5% for 2125 reflections collected on a Xentronics area detector. The drug is bound in the minor groove, at the five base-pair site 5'-ATTTG and is in a unique orientation. This is displaced by one base pair in the 5' direction compared to previously-determined structures of this drug with the sequence d(CGCGAATTCGCG)2. Reasons for this difference in behaviour are discussed in terms of several sequence-dependent structural features of the DNA, with particular reference to differences in propeller twist and minor-groove width.
About this Structure
296D is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Sequence-dependent effects in drug-DNA interaction: the crystal structure of Hoechst 33258 bound to the d(CGCAAATTTGCG)2 duplex., Spink N, Brown DG, Skelly JV, Neidle S, Nucleic Acids Res. 1994 May 11;22(9):1607-12. PMID:7515488
Page seeded by OCA on Thu Feb 21 16:22:17 2008
