2a25
From Proteopedia
(New page: 200px<br /> <applet load="2a25" size="450" color="white" frame="true" align="right" spinBox="true" caption="2a25, resolution 2.20Å" /> '''Crystal structure o...) |
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| - | [[Image:2a25.gif|left|200px]]<br /> | + | [[Image:2a25.gif|left|200px]]<br /><applet load="2a25" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2a25" size=" | + | |
caption="2a25, resolution 2.20Å" /> | caption="2a25, resolution 2.20Å" /> | ||
'''Crystal structure of Siah1 SBD bound to the peptide EKPAAVVAPITTG from SIP'''<br /> | '''Crystal structure of Siah1 SBD bound to the peptide EKPAAVVAPITTG from SIP'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Siah1 is the central component of a multiprotein E3 ubiquitin ligase | + | Siah1 is the central component of a multiprotein E3 ubiquitin ligase complex that targets beta-catenin for destruction in response to p53 activation. The E3 complex comprises, in addition to Siah1, Siah-interacting protein (SIP), the adaptor protein Skp1, and the F-box protein Ebi. Here we show that SIP engages Siah1 by means of two elements, both of which are required for mediating beta-catenin destruction in cells. An N-terminal dimerization domain of SIP sits across the saddle-shaped upper surface of Siah1, with two extended legs packing against the sides of Siah1 by means of a consensus PXAXVXP motif that is common to a family of Siah-binding proteins. The C-terminal domain of SIP, which binds to Skp1, protrudes from the lower surface of Siah1, and we propose that this surface provides the scaffold for bringing substrate and the E2 enzyme into apposition in the functional complex. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2A25 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2A25 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A25 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Ely, K | + | [[Category: Ely, K R.]] |
[[Category: Fukushima, T.]] | [[Category: Fukushima, T.]] | ||
[[Category: Leone, M.]] | [[Category: Leone, M.]] | ||
[[Category: Li, C.]] | [[Category: Li, C.]] | ||
| - | [[Category: Liddington, R | + | [[Category: Liddington, R C.]] |
[[Category: Matsuzawa, S.]] | [[Category: Matsuzawa, S.]] | ||
| - | [[Category: Olson, A | + | [[Category: Olson, A J.]] |
[[Category: Pellecchia, M.]] | [[Category: Pellecchia, M.]] | ||
| - | [[Category: Preece, N | + | [[Category: Preece, N E.]] |
| - | [[Category: Reed, J | + | [[Category: Reed, J C.]] |
[[Category: Santelli, E.]] | [[Category: Santelli, E.]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: protein-peptide complex]] | [[Category: protein-peptide complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:22:50 2008'' |
Revision as of 14:22, 21 February 2008
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Crystal structure of Siah1 SBD bound to the peptide EKPAAVVAPITTG from SIP
Contents |
Overview
Siah1 is the central component of a multiprotein E3 ubiquitin ligase complex that targets beta-catenin for destruction in response to p53 activation. The E3 complex comprises, in addition to Siah1, Siah-interacting protein (SIP), the adaptor protein Skp1, and the F-box protein Ebi. Here we show that SIP engages Siah1 by means of two elements, both of which are required for mediating beta-catenin destruction in cells. An N-terminal dimerization domain of SIP sits across the saddle-shaped upper surface of Siah1, with two extended legs packing against the sides of Siah1 by means of a consensus PXAXVXP motif that is common to a family of Siah-binding proteins. The C-terminal domain of SIP, which binds to Skp1, protrudes from the lower surface of Siah1, and we propose that this surface provides the scaffold for bringing substrate and the E2 enzyme into apposition in the functional complex.
Disease
Known disease associated with this structure: Mowat-Wilson syndrome OMIM:[605802]
About this Structure
2A25 is a Protein complex structure of sequences from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Structural analysis of Siah1-Siah-interacting protein interactions and insights into the assembly of an E3 ligase multiprotein complex., Santelli E, Leone M, Li C, Fukushima T, Preece NE, Olson AJ, Ely KR, Reed JC, Pellecchia M, Liddington RC, Matsuzawa S, J Biol Chem. 2005 Oct 7;280(40):34278-87. Epub 2005 Aug 4. PMID:16085652
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