2a3d
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Although de novo protein design is an important endeavor with implications | + | Although de novo protein design is an important endeavor with implications for understanding protein folding, until now, structures have been determined for only a few 25- to 30-residue designed miniproteins. Here, the NMR solution structure of a complex 73-residue three-helix bundle protein, alpha3D, is reported. The structure of alpha3D was not based on any natural protein, and yet it shows thermodynamic and spectroscopic properties typical of native proteins. A variety of features contribute to its unique structure, including electrostatics, the packing of a diverse set of hydrophobic side chains, and a loop that incorporates common capping motifs. Thus, it is now possible to design a complex protein with a well defined and predictable three-dimensional structure. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Bryson, J | + | [[Category: Bryson, J W.]] |
[[Category: Cheng, H.]] | [[Category: Cheng, H.]] | ||
| - | [[Category: Degrado, W | + | [[Category: Degrado, W F.]] |
[[Category: Roder, H.]] | [[Category: Roder, H.]] | ||
| - | [[Category: Walsh, S | + | [[Category: Walsh, S T.R.]] |
[[Category: three-helix bundle]] | [[Category: three-helix bundle]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:23:12 2008'' |
Revision as of 14:23, 21 February 2008
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SOLUTION STRUCTURE OF A DE NOVO DESIGNED SINGLE CHAIN THREE-HELIX BUNDLE (A3D)
Overview
Although de novo protein design is an important endeavor with implications for understanding protein folding, until now, structures have been determined for only a few 25- to 30-residue designed miniproteins. Here, the NMR solution structure of a complex 73-residue three-helix bundle protein, alpha3D, is reported. The structure of alpha3D was not based on any natural protein, and yet it shows thermodynamic and spectroscopic properties typical of native proteins. A variety of features contribute to its unique structure, including electrostatics, the packing of a diverse set of hydrophobic side chains, and a loop that incorporates common capping motifs. Thus, it is now possible to design a complex protein with a well defined and predictable three-dimensional structure.
About this Structure
2A3D is a Protein complex structure of sequences from Escherichia coli. The following page contains interesting information on the relation of 2A3D with [Designer Proteins]. Full crystallographic information is available from OCA.
Reference
Solution structure and dynamics of a de novo designed three-helix bundle protein., Walsh ST, Cheng H, Bryson JW, Roder H, DeGrado WF, Proc Natl Acad Sci U S A. 1999 May 11;96(10):5486-91. PMID:10318910
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