2a3r
From Proteopedia
(New page: 200px<br /> <applet load="2a3r" size="450" color="white" frame="true" align="right" spinBox="true" caption="2a3r, resolution 2.60Å" /> '''Crystal Structure o...) |
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caption="2a3r, resolution 2.60Å" /> | caption="2a3r, resolution 2.60Å" /> | ||
'''Crystal Structure of Human Sulfotransferase SULT1A3 in Complex with Dopamine and 3-Phosphoadenosine 5-Phosphate'''<br /> | '''Crystal Structure of Human Sulfotransferase SULT1A3 in Complex with Dopamine and 3-Phosphoadenosine 5-Phosphate'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The human sulfotransferase, SULT1A3, catalyzes specifically the | + | The human sulfotransferase, SULT1A3, catalyzes specifically the sulfonation of monoamines such as dopamine, epinephrine, and norepinephrine. SULT1A3 also has a unique 3,4-dihydroxyphenylalanine (Dopa)/tyrosine-sulfating activity that is preferentially toward their D-form enantiomers and can be stimulated dramatically by Mn2+. To further our understanding of the molecular basis for the unique substrate specificity of this enzyme, we solved the crystal structure of human SULT1A3, complexed with dopamine and 3'-phosphoadenosine 5'-phosphate, at 2.6 A resolution and carried out autodocking analysis with D-Dopa. The structure of SULT1A3 enzyme-ligand complex clearly showed that residue Glu146 can form electrostatic interaction with dopamine and may play a pivotal role in the stereoselectivity and sulfating activity. On the other hand, residue Asp86 appeared to be critical to the Mn2+-stimulation of the Dopa/tyrosine-sulfating activity of SULT1A3, in addition to a supporting role in the stereoselectivity and sulfating activity. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2A3R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with A3P and LDP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Aryl_sulfotransferase Aryl sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.1 2.8.2.1] Full crystallographic information is available from [http:// | + | 2A3R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=A3P:'>A3P</scene> and <scene name='pdbligand=LDP:'>LDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Aryl_sulfotransferase Aryl sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.1 2.8.2.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3R OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: An, X | + | [[Category: An, X M.]] |
| - | [[Category: Chang, W | + | [[Category: Chang, W R.]] |
| - | [[Category: Li, H | + | [[Category: Li, H T.]] |
[[Category: Li, M.]] | [[Category: Li, M.]] | ||
| - | [[Category: Liu, M | + | [[Category: Liu, M C.]] |
| - | [[Category: Lu, J | + | [[Category: Lu, J H.]] |
| - | [[Category: Zhang, J | + | [[Category: Zhang, J P.]] |
[[Category: A3P]] | [[Category: A3P]] | ||
[[Category: LDP]] | [[Category: LDP]] | ||
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[[Category: sult1a3]] | [[Category: sult1a3]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:23:21 2008'' |
Revision as of 14:23, 21 February 2008
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Crystal Structure of Human Sulfotransferase SULT1A3 in Complex with Dopamine and 3-Phosphoadenosine 5-Phosphate
Contents |
Overview
The human sulfotransferase, SULT1A3, catalyzes specifically the sulfonation of monoamines such as dopamine, epinephrine, and norepinephrine. SULT1A3 also has a unique 3,4-dihydroxyphenylalanine (Dopa)/tyrosine-sulfating activity that is preferentially toward their D-form enantiomers and can be stimulated dramatically by Mn2+. To further our understanding of the molecular basis for the unique substrate specificity of this enzyme, we solved the crystal structure of human SULT1A3, complexed with dopamine and 3'-phosphoadenosine 5'-phosphate, at 2.6 A resolution and carried out autodocking analysis with D-Dopa. The structure of SULT1A3 enzyme-ligand complex clearly showed that residue Glu146 can form electrostatic interaction with dopamine and may play a pivotal role in the stereoselectivity and sulfating activity. On the other hand, residue Asp86 appeared to be critical to the Mn2+-stimulation of the Dopa/tyrosine-sulfating activity of SULT1A3, in addition to a supporting role in the stereoselectivity and sulfating activity.
Disease
Known diseases associated with this structure: Alzheimer disease-4 OMIM:[600759], Cardiomyopathy, dilated, 1V OMIM:[600759]
About this Structure
2A3R is a Single protein structure of sequence from Homo sapiens with and as ligands. Active as Aryl sulfotransferase, with EC number 2.8.2.1 Full crystallographic information is available from OCA.
Reference
Crystal structure of human sulfotransferase SULT1A3 in complex with dopamine and 3'-phosphoadenosine 5'-phosphate., Lu JH, Li HT, Liu MC, Zhang JP, Li M, An XM, Chang WR, Biochem Biophys Res Commun. 2005 Sep 23;335(2):417-23. PMID:16083857
Page seeded by OCA on Thu Feb 21 16:23:21 2008
Categories: Aryl sulfotransferase | Homo sapiens | Single protein | An, X M. | Chang, W R. | Li, H T. | Li, M. | Liu, M C. | Lu, J H. | Zhang, J P. | A3P | LDP | Complex | Crystal structure | Dopamine | Sulfotransferase | Sult1a3
