2a43
From Proteopedia
(New page: 200px<br /><applet load="2a43" size="350" color="white" frame="true" align="right" spinBox="true" caption="2a43, resolution 1.34Å" /> '''Crystal Structure of...) |
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==Overview== | ==Overview== | ||
| - | To understand the role of structural elements of RNA pseudoknots in | + | To understand the role of structural elements of RNA pseudoknots in controlling the extent of -1-type ribosomal frameshifting, we determined the crystal structure of a high-efficiency frameshifting mutant of the pseudoknot from potato leaf roll virus (PLRV). Correlations of the structure with available in vitro frameshifting data for PLRV pseudoknot mutants implicate sequence and length of a stem-loop linker as modulators of frameshifting efficiency. Although the sequences and overall structures of the RNA pseudoknots from PLRV and beet western yellow virus (BWYV) are similar, nucleotide deletions in the linker and adjacent minor groove loop abolish frameshifting only with the latter. Conversely, mutant PLRV pseudoknots with up to four nucleotides deleted in this region exhibit nearly wild-type frameshifting efficiencies. The crystal structure helps rationalize the different tolerances for deletions in the PLRV and BWYV RNAs, and we have used it to build a three-dimensional model of the PRLV pseudoknot with a four-nucleotide deletion. The resulting structure defines a minimal RNA pseudoknot motif composed of 22 nucleotides capable of stimulating -1-type ribosomal frameshifts. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Egli, M.]] | [[Category: Egli, M.]] | ||
[[Category: Harp, J.]] | [[Category: Harp, J.]] | ||
| - | [[Category: II, B | + | [[Category: II, B A.Brown.]] |
| - | [[Category: III, F | + | [[Category: III, F C.Jewett.]] |
| - | [[Category: Marshall, W | + | [[Category: Marshall, W S.]] |
| - | [[Category: Pallan, P | + | [[Category: Pallan, P S.]] |
[[Category: Rich, A.]] | [[Category: Rich, A.]] | ||
[[Category: Wawrzak, Z.]] | [[Category: Wawrzak, Z.]] | ||
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[[Category: rna.]] | [[Category: rna.]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:23:24 2008'' |
Revision as of 14:23, 21 February 2008
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Crystal Structure of a Luteoviral RNA Pseudoknot and Model for a Minimal Ribosomal Frameshifting Motif
Overview
To understand the role of structural elements of RNA pseudoknots in controlling the extent of -1-type ribosomal frameshifting, we determined the crystal structure of a high-efficiency frameshifting mutant of the pseudoknot from potato leaf roll virus (PLRV). Correlations of the structure with available in vitro frameshifting data for PLRV pseudoknot mutants implicate sequence and length of a stem-loop linker as modulators of frameshifting efficiency. Although the sequences and overall structures of the RNA pseudoknots from PLRV and beet western yellow virus (BWYV) are similar, nucleotide deletions in the linker and adjacent minor groove loop abolish frameshifting only with the latter. Conversely, mutant PLRV pseudoknots with up to four nucleotides deleted in this region exhibit nearly wild-type frameshifting efficiencies. The crystal structure helps rationalize the different tolerances for deletions in the PLRV and BWYV RNAs, and we have used it to build a three-dimensional model of the PRLV pseudoknot with a four-nucleotide deletion. The resulting structure defines a minimal RNA pseudoknot motif composed of 22 nucleotides capable of stimulating -1-type ribosomal frameshifts.
About this Structure
2A43 is a Single protein structure of sequence from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of a luteoviral RNA pseudoknot and model for a minimal ribosomal frameshifting motif., Pallan PS, Marshall WS, Harp J, Jewett FC 3rd, Wawrzak Z, Brown BA 2nd, Rich A, Egli M, Biochemistry. 2005 Aug 30;44(34):11315-22. PMID:16114868
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