2a5d
From Proteopedia
(New page: 200px<br /> <applet load="2a5d" size="450" color="white" frame="true" align="right" spinBox="true" caption="2a5d, resolution 1.80Å" /> '''Structural basis fo...) |
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| - | [[Image:2a5d.gif|left|200px]]<br /> | + | [[Image:2a5d.gif|left|200px]]<br /><applet load="2a5d" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="2a5d, resolution 1.80Å" /> | caption="2a5d, resolution 1.80Å" /> | ||
'''Structural basis for the activation of cholera toxin by human ARF6-GTP'''<br /> | '''Structural basis for the activation of cholera toxin by human ARF6-GTP'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Vibrio cholerae bacterium causes devastating diarrhea when it infects | + | The Vibrio cholerae bacterium causes devastating diarrhea when it infects the human intestine. The key event is adenosine diphosphate (ADP)-ribosylation of the human signaling protein GSalpha, catalyzed by the cholera toxin A1 subunit (CTA1). This reaction is allosterically activated by human ADP-ribosylation factors (ARFs), a family of essential and ubiquitous G proteins. Crystal structures of a CTA1:ARF6-GTP (guanosine triphosphate) complex reveal that binding of the human activator elicits dramatic changes in CTA1 loop regions that allow nicotinamide adenine dinucleotide (NAD+) to bind to the active site. The extensive toxin:ARF-GTP interface surface mimics ARF-GTP recognition of normal cellular protein partners, which suggests that the toxin has evolved to exploit promiscuous binding properties of ARFs. |
==About this Structure== | ==About this Structure== | ||
| - | 2A5D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae] with NA, MG, GTP and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] Full crystallographic information is available from [http:// | + | 2A5D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae] with <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=GTP:'>GTP</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A5D OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Vibrio cholerae]] | [[Category: Vibrio cholerae]] | ||
| - | [[Category: Hol, W | + | [[Category: Hol, W G.J.]] |
| - | [[Category: Holmes, R | + | [[Category: Holmes, R K.]] |
| - | [[Category: Jobling, M | + | [[Category: Jobling, M G.]] |
| - | [[Category: Neal, C | + | [[Category: Neal, C J.O.]] |
[[Category: GOL]] | [[Category: GOL]] | ||
[[Category: GTP]] | [[Category: GTP]] | ||
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[[Category: protein transport/transferase]] | [[Category: protein transport/transferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:23:44 2008'' |
Revision as of 14:23, 21 February 2008
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Structural basis for the activation of cholera toxin by human ARF6-GTP
Overview
The Vibrio cholerae bacterium causes devastating diarrhea when it infects the human intestine. The key event is adenosine diphosphate (ADP)-ribosylation of the human signaling protein GSalpha, catalyzed by the cholera toxin A1 subunit (CTA1). This reaction is allosterically activated by human ADP-ribosylation factors (ARFs), a family of essential and ubiquitous G proteins. Crystal structures of a CTA1:ARF6-GTP (guanosine triphosphate) complex reveal that binding of the human activator elicits dramatic changes in CTA1 loop regions that allow nicotinamide adenine dinucleotide (NAD+) to bind to the active site. The extensive toxin:ARF-GTP interface surface mimics ARF-GTP recognition of normal cellular protein partners, which suggests that the toxin has evolved to exploit promiscuous binding properties of ARFs.
About this Structure
2A5D is a Protein complex structure of sequences from Homo sapiens and Vibrio cholerae with , , and as ligands. Active as NAD(+)--diphthamide ADP-ribosyltransferase, with EC number 2.4.2.36 Full crystallographic information is available from OCA.
Reference
Structural basis for the activation of cholera toxin by human ARF6-GTP., O'Neal CJ, Jobling MG, Holmes RK, Hol WG, Science. 2005 Aug 12;309(5737):1093-6. PMID:16099990
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