2a6q
From Proteopedia
(New page: 200px<br /><applet load="2a6q" size="450" color="white" frame="true" align="right" spinBox="true" caption="2a6q, resolution 2.05Å" /> '''Crystal structure of...) |
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| - | [[Image:2a6q.gif|left|200px]]<br /><applet load="2a6q" size=" | + | [[Image:2a6q.gif|left|200px]]<br /><applet load="2a6q" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2a6q, resolution 2.05Å" /> | caption="2a6q, resolution 2.05Å" /> | ||
'''Crystal structure of YefM-YoeB complex'''<br /> | '''Crystal structure of YefM-YoeB complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The eubacterial chromosome encodes various addiction modules that control | + | The eubacterial chromosome encodes various addiction modules that control global levels of translation through RNA degradation. Crystal structures of the Escherichia coli YefM2 (antitoxin)-YoeB (toxin) complex and the free YoeB toxin have been determined. The structure of the heterotrimeric complex reveals an asymmetric disorder-to-order recognition strategy, in which one C terminus of the YefM homodimer exclusively interacts with an atypical microbial ribonuclease (RNase) fold of YoeB. Comparison with the YefM-free YoeB structure indicates a conformational rearrangement of the RNase catalytic site of YoeB, induced by interaction with YefM. Complementary biochemical experiments demonstrate that the YoeB toxin has an in vitro RNase activity that preferentially cleaves at the 3' end of purine ribonucleotides. |
==About this Structure== | ==About this Structure== | ||
| - | 2A6Q is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 2A6Q is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A6Q OCA]. |
==Reference== | ==Reference== | ||
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[[Category: yoeb]] | [[Category: yoeb]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:24:06 2008'' |
Revision as of 14:24, 21 February 2008
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Crystal structure of YefM-YoeB complex
Overview
The eubacterial chromosome encodes various addiction modules that control global levels of translation through RNA degradation. Crystal structures of the Escherichia coli YefM2 (antitoxin)-YoeB (toxin) complex and the free YoeB toxin have been determined. The structure of the heterotrimeric complex reveals an asymmetric disorder-to-order recognition strategy, in which one C terminus of the YefM homodimer exclusively interacts with an atypical microbial ribonuclease (RNase) fold of YoeB. Comparison with the YefM-free YoeB structure indicates a conformational rearrangement of the RNase catalytic site of YoeB, induced by interaction with YefM. Complementary biochemical experiments demonstrate that the YoeB toxin has an in vitro RNase activity that preferentially cleaves at the 3' end of purine ribonucleotides.
About this Structure
2A6Q is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Conformational change in the catalytic site of the ribonuclease YoeB toxin by YefM antitoxin., Kamada K, Hanaoka F, Mol Cell. 2005 Aug 19;19(4):497-509. PMID:16109374
Page seeded by OCA on Thu Feb 21 16:24:06 2008
Categories: Escherichia coli | Protein complex | Hanaoka, F. | Kamada, K. | Addiction modules | Antitoxin | Inhibitor | Rnase | Toxin | Yefm | Yoeb
