2acf
From Proteopedia
(New page: 200px<br /><applet load="2acf" size="450" color="white" frame="true" align="right" spinBox="true" caption="2acf, resolution 1.40Å" /> '''Crystal structure of...) |
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| - | [[Image:2acf.gif|left|200px]]<br /><applet load="2acf" size=" | + | [[Image:2acf.gif|left|200px]]<br /><applet load="2acf" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2acf, resolution 1.40Å" /> | caption="2acf, resolution 1.40Å" /> | ||
'''Crystal structure of ADRP domain of SARS NSP3'''<br /> | '''Crystal structure of ADRP domain of SARS NSP3'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of a conserved domain of nonstructural protein 3 | + | The crystal structure of a conserved domain of nonstructural protein 3 (nsP3) from severe acute respiratory syndrome coronavirus (SARS-CoV) has been solved by single-wavelength anomalous dispersion to 1.4 A resolution. The structure of this "X" domain, seen in many single-stranded RNA viruses, reveals a three-layered alpha/beta/alpha core with a macro-H2A-like fold. The putative active site is a solvent-exposed cleft that is conserved in its three structural homologs, yeast Ymx7, Archeoglobus fulgidus AF1521, and Er58 from E. coli. Its sequence is similar to yeast YBR022W (also known as Poa1P), a known phosphatase that acts on ADP-ribose-1''-phosphate (Appr-1''-p). The SARS nsP3 domain readily removes the 1'' phosphate group from Appr-1''-p in in vitro assays, confirming its phosphatase activity. Sequence and structure comparison of all known macro-H2A domains combined with available functional data suggests that proteins of this superfamily form an emerging group of nucleotide phosphatases that dephosphorylate Appr-1''-p. |
==About this Structure== | ==About this Structure== | ||
| - | 2ACF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus] with GOL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2ACF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus] with <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ACF OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Sars coronavirus]] | [[Category: Sars coronavirus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Buchmeier, M | + | [[Category: Buchmeier, M J.]] |
| - | [[Category: JCSG, Joint | + | [[Category: JCSG, Joint Center for Structural Genomics.]] |
| - | [[Category: Joseph, J | + | [[Category: Joseph, J S.]] |
[[Category: Kuhn, P.]] | [[Category: Kuhn, P.]] | ||
| - | [[Category: Neuman, B | + | [[Category: Neuman, B W.]] |
| - | [[Category: Saikatendu, K | + | [[Category: Saikatendu, K S.]] |
| - | [[Category: Stevens, R | + | [[Category: Stevens, R C.]] |
[[Category: Subramanian, V.]] | [[Category: Subramanian, V.]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:25:58 2008'' |
Revision as of 14:26, 21 February 2008
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Crystal structure of ADRP domain of SARS NSP3
Overview
The crystal structure of a conserved domain of nonstructural protein 3 (nsP3) from severe acute respiratory syndrome coronavirus (SARS-CoV) has been solved by single-wavelength anomalous dispersion to 1.4 A resolution. The structure of this "X" domain, seen in many single-stranded RNA viruses, reveals a three-layered alpha/beta/alpha core with a macro-H2A-like fold. The putative active site is a solvent-exposed cleft that is conserved in its three structural homologs, yeast Ymx7, Archeoglobus fulgidus AF1521, and Er58 from E. coli. Its sequence is similar to yeast YBR022W (also known as Poa1P), a known phosphatase that acts on ADP-ribose-1-phosphate (Appr-1-p). The SARS nsP3 domain readily removes the 1 phosphate group from Appr-1-p in in vitro assays, confirming its phosphatase activity. Sequence and structure comparison of all known macro-H2A domains combined with available functional data suggests that proteins of this superfamily form an emerging group of nucleotide phosphatases that dephosphorylate Appr-1-p.
About this Structure
2ACF is a Single protein structure of sequence from Sars coronavirus with as ligand. Full crystallographic information is available from OCA.
Reference
Structural basis of severe acute respiratory syndrome coronavirus ADP-ribose-1-phosphate dephosphorylation by a conserved domain of nsP3., Saikatendu KS, Joseph JS, Subramanian V, Clayton T, Griffith M, Moy K, Velasquez J, Neuman BW, Buchmeier MJ, Stevens RC, Kuhn P, Structure. 2005 Nov;13(11):1665-75. PMID:16271890
Page seeded by OCA on Thu Feb 21 16:25:58 2008
Categories: Sars coronavirus | Single protein | Buchmeier, M J. | JCSG, Joint Center for Structural Genomics. | Joseph, J S. | Kuhn, P. | Neuman, B W. | Saikatendu, K S. | Stevens, R C. | Subramanian, V. | GOL | Adrp domain | Appr-1"-p phosphatase | Jcsg | Joint center for structural genomics | Protein structure initiative | Psi-2 | Sars nsp-3 | Structural genomics
