2ai8
From Proteopedia
(New page: 200px<br /><applet load="2ai8" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ai8, resolution 1.700Å" /> '''E.coli Polypeptide ...) |
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| - | [[Image:2ai8.jpg|left|200px]]<br /><applet load="2ai8" size=" | + | [[Image:2ai8.jpg|left|200px]]<br /><applet load="2ai8" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2ai8, resolution 1.700Å" /> | caption="2ai8, resolution 1.700Å" /> | ||
'''E.coli Polypeptide Deformylase complexed with SB-485343'''<br /> | '''E.coli Polypeptide Deformylase complexed with SB-485343'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Polypeptide deformylase (PDF) catalyzes the deformylation of polypeptide | + | Polypeptide deformylase (PDF) catalyzes the deformylation of polypeptide chains in bacteria. It is essential for bacterial cell viability and is a potential antibacterial drug target. Here, we report the crystal structures of polypeptide deformylase from four different species of bacteria: Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, and Escherichia coli. Comparison of these four structures reveals significant overall differences between the two Gram-negative species (E. coli and H. influenzae) and the two Gram-positive species (S. pneumoniae and S. aureus). Despite these differences and low overall sequence identity, the S1' pocket of PDF is well conserved among the four enzymes studied. We also describe the binding of nonpeptidic inhibitor molecules SB-485345, SB-543668, and SB-505684 to both S. pneumoniae and E. coli PDF. Comparison of these structures shows similar binding interactions with both Gram-negative and Gram-positive species. Understanding the similarities and subtle differences in active site structure between species will help to design broad-spectrum polypeptide deformylase inhibitor molecules. |
==About this Structure== | ==About this Structure== | ||
| - | 2AI8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with NI and SB7 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Peptide_deformylase Peptide deformylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.88 3.5.1.88] Full crystallographic information is available from [http:// | + | 2AI8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=NI:'>NI</scene> and <scene name='pdbligand=SB7:'>SB7</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Peptide_deformylase Peptide deformylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.88 3.5.1.88] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AI8 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Aubart, K.]] | [[Category: Aubart, K.]] | ||
| - | [[Category: Christensen, S | + | [[Category: Christensen, S B.]] |
[[Category: Fosberry, A.]] | [[Category: Fosberry, A.]] | ||
[[Category: Jones, J.]] | [[Category: Jones, J.]] | ||
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[[Category: Lonetto, M.]] | [[Category: Lonetto, M.]] | ||
[[Category: McManus, E.]] | [[Category: McManus, E.]] | ||
| - | [[Category: Petit, C | + | [[Category: Petit, C M.]] |
| - | [[Category: Smith, K | + | [[Category: Smith, K J.]] |
[[Category: Smyth, M.]] | [[Category: Smyth, M.]] | ||
[[Category: NI]] | [[Category: NI]] | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:27:40 2008'' |
Revision as of 14:27, 21 February 2008
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E.coli Polypeptide Deformylase complexed with SB-485343
Overview
Polypeptide deformylase (PDF) catalyzes the deformylation of polypeptide chains in bacteria. It is essential for bacterial cell viability and is a potential antibacterial drug target. Here, we report the crystal structures of polypeptide deformylase from four different species of bacteria: Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, and Escherichia coli. Comparison of these four structures reveals significant overall differences between the two Gram-negative species (E. coli and H. influenzae) and the two Gram-positive species (S. pneumoniae and S. aureus). Despite these differences and low overall sequence identity, the S1' pocket of PDF is well conserved among the four enzymes studied. We also describe the binding of nonpeptidic inhibitor molecules SB-485345, SB-543668, and SB-505684 to both S. pneumoniae and E. coli PDF. Comparison of these structures shows similar binding interactions with both Gram-negative and Gram-positive species. Understanding the similarities and subtle differences in active site structure between species will help to design broad-spectrum polypeptide deformylase inhibitor molecules.
About this Structure
2AI8 is a Single protein structure of sequence from Escherichia coli with and as ligands. Active as Peptide deformylase, with EC number 3.5.1.88 Full crystallographic information is available from OCA.
Reference
Structural variation and inhibitor binding in polypeptide deformylase from four different bacterial species., Smith KJ, Petit CM, Aubart K, Smyth M, McManus E, Jones J, Fosberry A, Lewis C, Lonetto M, Christensen SB, Protein Sci. 2003 Feb;12(2):349-60. PMID:12538898
Page seeded by OCA on Thu Feb 21 16:27:40 2008
Categories: Escherichia coli | Peptide deformylase | Single protein | Aubart, K. | Christensen, S B. | Fosberry, A. | Jones, J. | Lewis, C. | Lonetto, M. | McManus, E. | Petit, C M. | Smith, K J. | Smyth, M. | NI | SB7 | Hydrolase
