2aiq
From Proteopedia
(New page: 200px<br /><applet load="2aiq" size="450" color="white" frame="true" align="right" spinBox="true" caption="2aiq, resolution 1.54Å" /> '''Crystal structure of...) |
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| - | [[Image:2aiq.gif|left|200px]]<br /><applet load="2aiq" size=" | + | [[Image:2aiq.gif|left|200px]]<br /><applet load="2aiq" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2aiq, resolution 1.54Å" /> | caption="2aiq, resolution 1.54Å" /> | ||
'''Crystal structure of benzamidine-inhibited protein C activator from the venom of copperhead snake Agkistrodon contortrix contortrix'''<br /> | '''Crystal structure of benzamidine-inhibited protein C activator from the venom of copperhead snake Agkistrodon contortrix contortrix'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Protein C activation initiated by the thrombin-thrombomodulin complex | + | Protein C activation initiated by the thrombin-thrombomodulin complex forms the major physiological anticoagulant pathway. Agkistrodon contortrix contortrix protein C activator, a glycosylated single-chain serine proteinase, activates protein C without relying on thrombomodulin. The crystal structures of native and inhibited Agkistrodon contortrix contortrix protein C activator determined at 1.65 and 1.54 A resolutions, respectively, indicate the pivotal roles played by the positively charged belt and the strategic positioning of the three carbohydrate moieties surrounding the catalytic site in protein C recognition, binding, and activation. Structural changes in the benzamidine-inhibited enzyme suggest a probable function in allosteric regulation for the anion-binding site located in the C-terminal extension, which is fully conserved in snake venom serine proteinases, that preferentially binds Cl(1-) instead of SO(4)(2-). |
==About this Structure== | ==About this Structure== | ||
| - | 2AIQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Agkistrodon_contortrix_contortrix Agkistrodon contortrix contortrix] with NAG, NDG, SO4, ACT, CL, BEN and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Venombin_A Venombin A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.74 3.4.21.74] Full crystallographic information is available from [http:// | + | 2AIQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Agkistrodon_contortrix_contortrix Agkistrodon contortrix contortrix] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=NDG:'>NDG</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=ACT:'>ACT</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=BEN:'>BEN</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Venombin_A Venombin A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.74 3.4.21.74] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AIQ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Venombin A]] | [[Category: Venombin A]] | ||
| - | [[Category: Arni, R | + | [[Category: Arni, R K.]] |
| - | [[Category: Murakami, M | + | [[Category: Murakami, M T.]] |
[[Category: ACT]] | [[Category: ACT]] | ||
[[Category: BEN]] | [[Category: BEN]] | ||
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[[Category: snake venom serine proteinase]] | [[Category: snake venom serine proteinase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:27:49 2008'' |
Revision as of 14:27, 21 February 2008
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Crystal structure of benzamidine-inhibited protein C activator from the venom of copperhead snake Agkistrodon contortrix contortrix
Overview
Protein C activation initiated by the thrombin-thrombomodulin complex forms the major physiological anticoagulant pathway. Agkistrodon contortrix contortrix protein C activator, a glycosylated single-chain serine proteinase, activates protein C without relying on thrombomodulin. The crystal structures of native and inhibited Agkistrodon contortrix contortrix protein C activator determined at 1.65 and 1.54 A resolutions, respectively, indicate the pivotal roles played by the positively charged belt and the strategic positioning of the three carbohydrate moieties surrounding the catalytic site in protein C recognition, binding, and activation. Structural changes in the benzamidine-inhibited enzyme suggest a probable function in allosteric regulation for the anion-binding site located in the C-terminal extension, which is fully conserved in snake venom serine proteinases, that preferentially binds Cl(1-) instead of SO(4)(2-).
About this Structure
2AIQ is a Single protein structure of sequence from Agkistrodon contortrix contortrix with , , , , , and as ligands. Active as Venombin A, with EC number 3.4.21.74 Full crystallographic information is available from OCA.
Reference
Thrombomodulin-independent activation of protein C and specificity of hemostatically active snake venom serine proteinases: crystal structures of native and inhibited Agkistrodon contortrix contortrix protein C activator., Murakami MT, Arni RK, J Biol Chem. 2005 Nov 25;280(47):39309-15. Epub 2005 Sep 14. PMID:16162508
Page seeded by OCA on Thu Feb 21 16:27:49 2008
Categories: Agkistrodon contortrix contortrix | Single protein | Venombin A | Arni, R K. | Murakami, M T. | ACT | BEN | CL | GOL | NAG | NDG | SO4 | Protein c activator | Snake venom serine proteinase
