2akr
From Proteopedia
(New page: 200px<br /><applet load="2akr" size="450" color="white" frame="true" align="right" spinBox="true" caption="2akr, resolution 1.90Å" /> '''Structural basis of ...) |
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| - | [[Image:2akr.gif|left|200px]]<br /><applet load="2akr" size=" | + | [[Image:2akr.gif|left|200px]]<br /><applet load="2akr" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2akr, resolution 1.90Å" /> | caption="2akr, resolution 1.90Å" /> | ||
'''Structural basis of sulfatide presentation by mouse CD1d'''<br /> | '''Structural basis of sulfatide presentation by mouse CD1d'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Sulfatide derived from the myelin stimulates a distinct population of | + | Sulfatide derived from the myelin stimulates a distinct population of CD1d-restricted natural killer T (NKT) cells. Cis-tetracosenoyl sulfatide is one of the immunodominant species in myelin as identified by proliferation, cytokine secretion, and CD1d tetramer staining. The crystal structure of mouse CD1d in complex with cis-tetracosenoyl sulfatide at 1.9 A resolution reveals that the longer cis-tetracosenoyl fatty acid chain fully occupies the A' pocket of the CD1d binding groove, whereas the sphingosine chain fills up the F' pocket. A precise hydrogen bond network in the center of the binding groove orients and positions the ceramide backbone for insertion of the lipid tails in their respective pockets. The 3'-sulfated galactose headgroup is highly exposed for presentation to the T cell receptor and projects up and away from the binding pocket due to its beta linkage, compared with the more intimate binding of the alpha-glactosyl ceramide headgroup to CD1d. These structure and binding data on sulfatide presentation by CD1d have important implications for the design of therapeutics that target T cells reactive for myelin glycolipids in autoimmune diseases of the central nervous system. |
==About this Structure== | ==About this Structure== | ||
| - | 2AKR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with NAG and CIS as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2AKR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=NAG:'>NAG</scene> and <scene name='pdbligand=CIS:'>CIS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AKR OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Maricic, I.]] | [[Category: Maricic, I.]] | ||
[[Category: Roy, K.]] | [[Category: Roy, K.]] | ||
| - | [[Category: Wilson, I | + | [[Category: Wilson, I A.]] |
| - | [[Category: Wong, C | + | [[Category: Wong, C H.]] |
[[Category: Wu, D.]] | [[Category: Wu, D.]] | ||
| - | [[Category: Zajonc, D | + | [[Category: Zajonc, D M.]] |
[[Category: CIS]] | [[Category: CIS]] | ||
[[Category: NAG]] | [[Category: NAG]] | ||
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[[Category: tcr]] | [[Category: tcr]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:28:23 2008'' |
Revision as of 14:28, 21 February 2008
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Structural basis of sulfatide presentation by mouse CD1d
Overview
Sulfatide derived from the myelin stimulates a distinct population of CD1d-restricted natural killer T (NKT) cells. Cis-tetracosenoyl sulfatide is one of the immunodominant species in myelin as identified by proliferation, cytokine secretion, and CD1d tetramer staining. The crystal structure of mouse CD1d in complex with cis-tetracosenoyl sulfatide at 1.9 A resolution reveals that the longer cis-tetracosenoyl fatty acid chain fully occupies the A' pocket of the CD1d binding groove, whereas the sphingosine chain fills up the F' pocket. A precise hydrogen bond network in the center of the binding groove orients and positions the ceramide backbone for insertion of the lipid tails in their respective pockets. The 3'-sulfated galactose headgroup is highly exposed for presentation to the T cell receptor and projects up and away from the binding pocket due to its beta linkage, compared with the more intimate binding of the alpha-glactosyl ceramide headgroup to CD1d. These structure and binding data on sulfatide presentation by CD1d have important implications for the design of therapeutics that target T cells reactive for myelin glycolipids in autoimmune diseases of the central nervous system.
About this Structure
2AKR is a Protein complex structure of sequences from Mus musculus with and as ligands. Full crystallographic information is available from OCA.
Reference
Structural basis for CD1d presentation of a sulfatide derived from myelin and its implications for autoimmunity., Zajonc DM, Maricic I, Wu D, Halder R, Roy K, Wong CH, Kumar V, Wilson IA, J Exp Med. 2005 Dec 5;202(11):1517-26. Epub 2005 Nov 28. PMID:16314439
Page seeded by OCA on Thu Feb 21 16:28:23 2008
Categories: Mus musculus | Protein complex | Halder, R. | Kumar, V. | Maricic, I. | Roy, K. | Wilson, I A. | Wong, C H. | Wu, D. | Zajonc, D M. | CIS | NAG | Cd1d | Mhc fold | Nkt cells | Self-antigen | Sulfatide | Tcr
