2an6
From Proteopedia
(New page: 200px<br /><applet load="2an6" size="450" color="white" frame="true" align="right" spinBox="true" caption="2an6, resolution 3.00Å" /> '''Protein-peptide comp...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2an6.gif|left|200px]]<br /><applet load="2an6" size=" | + | [[Image:2an6.gif|left|200px]]<br /><applet load="2an6" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2an6, resolution 3.00Å" /> | caption="2an6, resolution 3.00Å" /> | ||
'''Protein-peptide complex'''<br /> | '''Protein-peptide complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Siah family of RING proteins function as ubiquitin ligase components, contributing to the degradation of multiple targets involved in cell | + | The Siah family of RING proteins function as ubiquitin ligase components, contributing to the degradation of multiple targets involved in cell growth, differentiation, angiogenesis, oncogenesis, and inflammation. Previously, a binding motif (degron) was recognized in many of the Siah degradation targets, suggesting that Siah itself may facilitate substrate recognition. We report the crystal structure of the Siah in complex with a peptide containing the degron motif. Binding is within a groove formed in part by the zinc fingers and the first two beta strands of the TRAF-C domain of Siah. We show that residues in the degron, previously described to facilitate binding to Siah, interact with the protein. Mutagenesis of Siah at sites of interaction also abrogates both in vitro peptide binding and destabilization of a known Siah target. |
==About this Structure== | ==About this Structure== | ||
| - | 2AN6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2AN6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AN6 OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bowtell, D | + | [[Category: Bowtell, D D.L.]] |
| - | [[Category: Cromer, B | + | [[Category: Cromer, B A.]] |
[[Category: Fedorov, V.]] | [[Category: Fedorov, V.]] | ||
| - | [[Category: Hancock, N | + | [[Category: Hancock, N C.]] |
| - | [[Category: House, C | + | [[Category: House, C M.]] |
[[Category: Moller, A.]] | [[Category: Moller, A.]] | ||
| - | [[Category: Parker, M | + | [[Category: Parker, M W.]] |
[[Category: Polekhina, G.]] | [[Category: Polekhina, G.]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: protein-peptide complex]] | [[Category: protein-peptide complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:28:59 2008'' |
Revision as of 14:29, 21 February 2008
|
Protein-peptide complex
Overview
The Siah family of RING proteins function as ubiquitin ligase components, contributing to the degradation of multiple targets involved in cell growth, differentiation, angiogenesis, oncogenesis, and inflammation. Previously, a binding motif (degron) was recognized in many of the Siah degradation targets, suggesting that Siah itself may facilitate substrate recognition. We report the crystal structure of the Siah in complex with a peptide containing the degron motif. Binding is within a groove formed in part by the zinc fingers and the first two beta strands of the TRAF-C domain of Siah. We show that residues in the degron, previously described to facilitate binding to Siah, interact with the protein. Mutagenesis of Siah at sites of interaction also abrogates both in vitro peptide binding and destabilization of a known Siah target.
About this Structure
2AN6 is a Single protein structure of sequence from Mus musculus with as ligand. Full crystallographic information is available from OCA.
Reference
Elucidation of the substrate binding site of Siah ubiquitin ligase., House CM, Hancock NC, Moller A, Cromer BA, Fedorov V, Bowtell DD, Parker MW, Polekhina G, Structure. 2006 Apr;14(4):695-701. PMID:16615911
Page seeded by OCA on Thu Feb 21 16:28:59 2008
