2aql
From Proteopedia
(New page: 200px<br /> <applet load="2aql" size="450" color="white" frame="true" align="right" spinBox="true" caption="2aql, resolution 2.30Å" /> '''Crystal Structure o...) |
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| - | [[Image:2aql.gif|left|200px]]<br /> | + | [[Image:2aql.gif|left|200px]]<br /><applet load="2aql" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="2aql, resolution 2.30Å" /> | caption="2aql, resolution 2.30Å" /> | ||
'''Crystal Structure of the MRG15 MRG domain'''<br /> | '''Crystal Structure of the MRG15 MRG domain'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The ubiquitous MRG/MORF family of proteins is involved in cell senescence, or the terminal loss of proliferative potential, a model for aging and | + | The ubiquitous MRG/MORF family of proteins is involved in cell senescence, or the terminal loss of proliferative potential, a model for aging and tumor suppression at the cellular level. These proteins are defined by the approximately 20 kDa MRG domain that binds a plethora of transcriptional regulators and chromatin-remodeling factors, including the histone deacetylase transcriptional corepressor mSin3A and the novel nuclear protein PAM14, and they are also known components of the Tip60/NuA4 complex via interactions with the MRG binding protein (MRGBP). We present here the crystal structure of a prototypic MRG domain from human MRG15 whose core consists of two orthogonal helix hairpins. Despite the lack of sequence similarity, the core structure has surprisingly striking homology to a DNA-interacting domain of the tyrosine site-specific recombinases XerD, lambda integrase, and Cre. Site-directed mutagenesis studies based on the X-ray structure and bioinformatics identified key residues involved in the binding of PAM14 and MRGBP. |
==About this Structure== | ==About this Structure== | ||
| - | 2AQL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 2AQL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AQL OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bowman, B | + | [[Category: Bowman, B R.]] |
| - | [[Category: Quiocho, F | + | [[Category: Quiocho, F A.]] |
[[Category: chromatin remodeling]] | [[Category: chromatin remodeling]] | ||
[[Category: core binding domain]] | [[Category: core binding domain]] | ||
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[[Category: recombinase]] | [[Category: recombinase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:29:52 2008'' |
Revision as of 14:29, 21 February 2008
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Crystal Structure of the MRG15 MRG domain
Overview
The ubiquitous MRG/MORF family of proteins is involved in cell senescence, or the terminal loss of proliferative potential, a model for aging and tumor suppression at the cellular level. These proteins are defined by the approximately 20 kDa MRG domain that binds a plethora of transcriptional regulators and chromatin-remodeling factors, including the histone deacetylase transcriptional corepressor mSin3A and the novel nuclear protein PAM14, and they are also known components of the Tip60/NuA4 complex via interactions with the MRG binding protein (MRGBP). We present here the crystal structure of a prototypic MRG domain from human MRG15 whose core consists of two orthogonal helix hairpins. Despite the lack of sequence similarity, the core structure has surprisingly striking homology to a DNA-interacting domain of the tyrosine site-specific recombinases XerD, lambda integrase, and Cre. Site-directed mutagenesis studies based on the X-ray structure and bioinformatics identified key residues involved in the binding of PAM14 and MRGBP.
About this Structure
2AQL is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Multipurpose MRG domain involved in cell senescence and proliferation exhibits structural homology to a DNA-interacting domain., Bowman BR, Moure CM, Kirtane BM, Welschhans RL, Tominaga K, Pereira-Smith OM, Quiocho FA, Structure. 2006 Jan;14(1):151-8. PMID:16407074
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