2arl
From Proteopedia
(New page: 200px<br /><applet load="2arl" size="450" color="white" frame="true" align="right" spinBox="true" caption="2arl, resolution 2.0Å" /> '''The 2.0 angstroms cry...) |
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| - | [[Image:2arl.jpg|left|200px]]<br /><applet load="2arl" size=" | + | [[Image:2arl.jpg|left|200px]]<br /><applet load="2arl" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2arl, resolution 2.0Å" /> | caption="2arl, resolution 2.0Å" /> | ||
'''The 2.0 angstroms crystal structure of a pocilloporin at pH 3.5: the structural basis for the linkage between color transition and halide binding'''<br /> | '''The 2.0 angstroms crystal structure of a pocilloporin at pH 3.5: the structural basis for the linkage between color transition and halide binding'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The pocilloporin Rtms5 and an engineered variant Rtms5H146S undergo | + | The pocilloporin Rtms5 and an engineered variant Rtms5H146S undergo distinct color transitions (from blue to red to yellow to colorless) in a pH-dependent manner. pK(a) values of 4.1 and 3.2 were determined for the blue (absorption lambda(max), 590 nm) to yellow (absorption lambda(max), approximately 453 nm) transitions of Rtms5 and Rtms5H146. The pK(a) for the blue-yellow transition of Rtms5H146S increased by 1.4 U in the presence of 0.1 M KI, whereas the pK(a) for the same transition of Rtms5 was relatively insensitive to added halides. To understand the structural basis for these observations, we have determined to 2.0 angstroms resolution the crystal structure of a yellow form of Rtms5H146S at pH 3.5 in the presence of iodide. Iodide was found occupying a pocket in the structure with a pH of 3.5, forming van der Waals contacts with the tyrosyl moiety of the chromophore. Elsewhere, it was determined that this pocket is occupied by a water molecule in the Rtms5H146S structure (pH 8.0) and by the side chain of histidine 146 in the wild-type Rtms5 structure. Collectively, our data provide an explanation for the observed linkage between color transitions for Rtms5H146S and binding to halides. |
==About this Structure== | ==About this Structure== | ||
| - | 2ARL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Montipora_efflorescens Montipora efflorescens] with IOD, CL and ACY as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2ARL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Montipora_efflorescens Montipora efflorescens] with <scene name='pdbligand=IOD:'>IOD</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=ACY:'>ACY</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ARL OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Battad, J.]] | [[Category: Battad, J.]] | ||
[[Category: Beddoe, T.]] | [[Category: Beddoe, T.]] | ||
| - | [[Category: Devenish, R | + | [[Category: Devenish, R J.]] |
[[Category: Dove, S.]] | [[Category: Dove, S.]] | ||
[[Category: Olsen, S.]] | [[Category: Olsen, S.]] | ||
[[Category: Prescott, M.]] | [[Category: Prescott, M.]] | ||
[[Category: Rossjohn, J.]] | [[Category: Rossjohn, J.]] | ||
| - | [[Category: Smith, S | + | [[Category: Smith, S C.]] |
| - | [[Category: Wilmann, P | + | [[Category: Wilmann, P G.]] |
[[Category: ACY]] | [[Category: ACY]] | ||
[[Category: CL]] | [[Category: CL]] | ||
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[[Category: luminescence]] | [[Category: luminescence]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:30:16 2008'' |
Revision as of 14:30, 21 February 2008
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The 2.0 angstroms crystal structure of a pocilloporin at pH 3.5: the structural basis for the linkage between color transition and halide binding
Overview
The pocilloporin Rtms5 and an engineered variant Rtms5H146S undergo distinct color transitions (from blue to red to yellow to colorless) in a pH-dependent manner. pK(a) values of 4.1 and 3.2 were determined for the blue (absorption lambda(max), 590 nm) to yellow (absorption lambda(max), approximately 453 nm) transitions of Rtms5 and Rtms5H146. The pK(a) for the blue-yellow transition of Rtms5H146S increased by 1.4 U in the presence of 0.1 M KI, whereas the pK(a) for the same transition of Rtms5 was relatively insensitive to added halides. To understand the structural basis for these observations, we have determined to 2.0 angstroms resolution the crystal structure of a yellow form of Rtms5H146S at pH 3.5 in the presence of iodide. Iodide was found occupying a pocket in the structure with a pH of 3.5, forming van der Waals contacts with the tyrosyl moiety of the chromophore. Elsewhere, it was determined that this pocket is occupied by a water molecule in the Rtms5H146S structure (pH 8.0) and by the side chain of histidine 146 in the wild-type Rtms5 structure. Collectively, our data provide an explanation for the observed linkage between color transitions for Rtms5H146S and binding to halides.
About this Structure
2ARL is a Single protein structure of sequence from Montipora efflorescens with , and as ligands. Full crystallographic information is available from OCA.
Reference
The 2.0 angstroms crystal structure of a pocilloporin at pH 3.5: the structural basis for the linkage between color transition and halide binding., Wilmann PG, Battad J, Beddoe T, Olsen S, Smith SC, Dove S, Devenish RJ, Rossjohn J, Prescott M, Photochem Photobiol. 2006 Mar-Apr;82(2):359-66. PMID:16613486
Page seeded by OCA on Thu Feb 21 16:30:16 2008
Categories: Montipora efflorescens | Single protein | Battad, J. | Beddoe, T. | Devenish, R J. | Dove, S. | Olsen, S. | Prescott, M. | Rossjohn, J. | Smith, S C. | Wilmann, P G. | ACY | CL | IOD | Chromophore | Luminescence
