2atx
From Proteopedia
(New page: 200px<br /> <applet load="2atx" size="450" color="white" frame="true" align="right" spinBox="true" caption="2atx, resolution 2.65Å" /> '''Crystal Structure o...) |
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| - | [[Image:2atx.gif|left|200px]]<br /> | + | [[Image:2atx.gif|left|200px]]<br /><applet load="2atx" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2atx" size=" | + | |
caption="2atx, resolution 2.65Å" /> | caption="2atx, resolution 2.65Å" /> | ||
'''Crystal Structure of the TC10 GppNHp complex'''<br /> | '''Crystal Structure of the TC10 GppNHp complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The specific and rapid formation of protein complexes is essential for | + | The specific and rapid formation of protein complexes is essential for diverse cellular processes such as remodeling of actin filaments in response to the interaction between Rho GTPases and the Wiskott-Aldrich syndrome proteins (WASp and N-WASp). Although Cdc42, TC10, and other members of the Rho family have been implicated in binding to and activating the WAS proteins, the exact nature of such a protein-protein recognition process has remained obscure. Here, we describe a mechanism that ensures rapid and selective long-range Cdc42-WASp recognition. The crystal structure of TC10, together with mutational and bioinformatic analyses, proved that the basic region of WASp and two unique glutamates in Cdc42 generate favorable electrostatic steering forces that control the accelerated WASp-Cdc42 association reaction. This process is a prerequisite for WASp activation and a critical step in temporal regulation and integration of WASp-mediated cellular responses. |
==About this Structure== | ==About this Structure== | ||
| - | 2ATX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG and GNP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Small_monomeric_GTPase Small monomeric GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.2 3.6.5.2] Full crystallographic information is available from [http:// | + | 2ATX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GNP:'>GNP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Small_monomeric_GTPase Small monomeric GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.2 3.6.5.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ATX OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Small monomeric GTPase]] | [[Category: Small monomeric GTPase]] | ||
| - | [[Category: Ahmadian, M | + | [[Category: Ahmadian, M R.]] |
| - | [[Category: Carlier, M | + | [[Category: Carlier, M F.]] |
[[Category: Dvorsky, R.]] | [[Category: Dvorsky, R.]] | ||
[[Category: Fiegen, D.]] | [[Category: Fiegen, D.]] | ||
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[[Category: tc10]] | [[Category: tc10]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:30:57 2008'' |
Revision as of 14:31, 21 February 2008
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Crystal Structure of the TC10 GppNHp complex
Overview
The specific and rapid formation of protein complexes is essential for diverse cellular processes such as remodeling of actin filaments in response to the interaction between Rho GTPases and the Wiskott-Aldrich syndrome proteins (WASp and N-WASp). Although Cdc42, TC10, and other members of the Rho family have been implicated in binding to and activating the WAS proteins, the exact nature of such a protein-protein recognition process has remained obscure. Here, we describe a mechanism that ensures rapid and selective long-range Cdc42-WASp recognition. The crystal structure of TC10, together with mutational and bioinformatic analyses, proved that the basic region of WASp and two unique glutamates in Cdc42 generate favorable electrostatic steering forces that control the accelerated WASp-Cdc42 association reaction. This process is a prerequisite for WASp activation and a critical step in temporal regulation and integration of WASp-mediated cellular responses.
About this Structure
2ATX is a Single protein structure of sequence from Homo sapiens with and as ligands. Active as Small monomeric GTPase, with EC number 3.6.5.2 Full crystallographic information is available from OCA.
Reference
An electrostatic steering mechanism of Cdc42 recognition by Wiskott-Aldrich syndrome proteins., Hemsath L, Dvorsky R, Fiegen D, Carlier MF, Ahmadian MR, Mol Cell. 2005 Oct 28;20(2):313-24. PMID:16246732
Page seeded by OCA on Thu Feb 21 16:30:57 2008
Categories: Homo sapiens | Single protein | Small monomeric GTPase | Ahmadian, M R. | Carlier, M F. | Dvorsky, R. | Fiegen, D. | Hemsath, L. | GNP | MG | Alpha-beta | Gtpase | P-loop | Tc10
