1myr

From Proteopedia

Revision as of 13:38, 30 October 2007

MYROSINASE FROM SINAPIS ALBA

Overview

BACKGROUND: Myrosinase is the enzyme responsible for the hydrolysis of a, variety of plant anionic 1-thio-beta-D-glucosides called glucosinolates., Myrosinase and glucosinolates, which are stored in different tissues of, the plant, are mixed during mastication generating toxic by-products that, are believed to play a role in the plant defence system. Whilst, O-glycosidases are extremely widespread in nature, myrosinase is the only, known S-glycosidase. This intriguing enzyme, which shows sequence, similarities with O-glycosidases, offers the opportunity to analyze the, similarities and differences between enzymes hydrolyzing S- and, O-glycosidic bonds. RESULTS: The structures of native myrosinase from, white mustard seed (Sinapis alba) and of a stable glycosyl-enzyme, intermediate have ... [(full description)]

About this Structure

1MYR is a [Protein complex] structure of sequences from [Sinapis alba] with NAG, NDG, ZN, SO4 and GOL as [ligands]. Active as [Transferred entry: 3.2.1.147], with EC number [3.2.3.1]. Structure known Active Site: ZNB. Full crystallographic information is available from [OCA].

Reference

The crystal structures of Sinapis alba myrosinase and a covalent glycosyl-enzyme intermediate provide insights into the substrate recognition and active-site machinery of an S-glycosidase., Burmeister WP, Cottaz S, Driguez H, Iori R, Palmieri S, Henrissat B, Structure. 1997 May 15;5(5):663-75. PMID:9195886

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