2avu
From Proteopedia
(New page: 200px<br /><applet load="2avu" size="450" color="white" frame="true" align="right" spinBox="true" caption="2avu, resolution 3.00Å" /> '''Structure of the Esc...) |
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| - | [[Image:2avu.gif|left|200px]]<br /><applet load="2avu" size=" | + | [[Image:2avu.gif|left|200px]]<br /><applet load="2avu" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2avu, resolution 3.00Å" /> | caption="2avu, resolution 3.00Å" /> | ||
'''Structure of the Escherichia coli FlhDC complex, a prokaryotic heteromeric regulator of transcription'''<br /> | '''Structure of the Escherichia coli FlhDC complex, a prokaryotic heteromeric regulator of transcription'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The hetero-oligomeric complex of the FlhD and FlhC proteins (FlhDC) | + | The hetero-oligomeric complex of the FlhD and FlhC proteins (FlhDC) regulates transcription from several flagellar and non-flagellar operons in bacteria. The crystallographic structure of the Escherichia coli FlhDC complex has been solved to 3.0 A resolution, revealing a hexameric FlhD4FlhC2 assembly. In the complex, each FlhC protomer binds an FlhD2 dimer; the conformation of the dimer in the complex differs significantly from its conformation in the absence of FlhC. FlhC has a novel tertiary fold that includes a heretofore unrecognized zinc-binding site in which the ion is ligated by four cysteine residues. Gel shift experiments show that binding of the FlhDC complex to a cognate promoter bends the DNA by approximately 111 degrees . The structure of the FlhDC complex is compatible with models in which a fragment of operator DNA, at least 48 base-pairs in length, wraps around the complex and bends significantly when binding. |
==About this Structure== | ==About this Structure== | ||
| - | 2AVU is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2AVU is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AVU OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Fleming, R | + | [[Category: Fleming, R T.]] |
[[Category: Matsumura, P.]] | [[Category: Matsumura, P.]] | ||
| - | [[Category: McKay, D | + | [[Category: McKay, D B.]] |
[[Category: Wang, S.]] | [[Category: Wang, S.]] | ||
| - | [[Category: Westbrook, E | + | [[Category: Westbrook, E M.]] |
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: c4-type zinc finger]] | [[Category: c4-type zinc finger]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:31:29 2008'' |
Revision as of 14:31, 21 February 2008
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Structure of the Escherichia coli FlhDC complex, a prokaryotic heteromeric regulator of transcription
Overview
The hetero-oligomeric complex of the FlhD and FlhC proteins (FlhDC) regulates transcription from several flagellar and non-flagellar operons in bacteria. The crystallographic structure of the Escherichia coli FlhDC complex has been solved to 3.0 A resolution, revealing a hexameric FlhD4FlhC2 assembly. In the complex, each FlhC protomer binds an FlhD2 dimer; the conformation of the dimer in the complex differs significantly from its conformation in the absence of FlhC. FlhC has a novel tertiary fold that includes a heretofore unrecognized zinc-binding site in which the ion is ligated by four cysteine residues. Gel shift experiments show that binding of the FlhDC complex to a cognate promoter bends the DNA by approximately 111 degrees . The structure of the FlhDC complex is compatible with models in which a fragment of operator DNA, at least 48 base-pairs in length, wraps around the complex and bends significantly when binding.
About this Structure
2AVU is a Protein complex structure of sequences from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of the Escherichia coli FlhDC complex, a prokaryotic heteromeric regulator of transcription., Wang S, Fleming RT, Westbrook EM, Matsumura P, McKay DB, J Mol Biol. 2006 Jan 27;355(4):798-808. Epub 2005 Nov 22. PMID:16337229
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