2az2
From Proteopedia
(New page: 200px<br /><applet load="2az2" size="450" color="white" frame="true" align="right" spinBox="true" caption="2az2, resolution 2.60Å" /> '''Flock House virus B2...) |
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| - | [[Image:2az2.gif|left|200px]]<br /><applet load="2az2" size=" | + | [[Image:2az2.gif|left|200px]]<br /><applet load="2az2" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2az2, resolution 2.60Å" /> | caption="2az2, resolution 2.60Å" /> | ||
'''Flock House virus B2-dsRNA Complex (P4122)'''<br /> | '''Flock House virus B2-dsRNA Complex (P4122)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | As a counter-defense against antiviral RNA silencing during infection, the | + | As a counter-defense against antiviral RNA silencing during infection, the insect Flock House virus (FHV) expresses the silencing suppressor protein B2. Biochemical experiments show that B2 binds to double-stranded RNA (dsRNA) without regard to length and inhibits cleavage of dsRNA by Dicer in vitro. A cocrystal structure reveals that a B2 dimer forms a four-helix bundle that binds to one face of an A-form RNA duplex independently of sequence. These results suggest that B2 blocks both cleavage of the FHV genome by Dicer and incorporation of FHV small interfering RNAs into the RNA-induced silencing complex. |
==About this Structure== | ==About this Structure== | ||
| - | 2AZ2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Flock_house_virus Flock house virus]. Full crystallographic information is available from [http:// | + | 2AZ2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Flock_house_virus Flock house virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AZ2 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Flock house virus]] | [[Category: Flock house virus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chao, J | + | [[Category: Chao, J A.]] |
| - | [[Category: Chapados, B | + | [[Category: Chapados, B R.]] |
| - | [[Category: Debler, E | + | [[Category: Debler, E W.]] |
| - | [[Category: Lee, J | + | [[Category: Lee, J H.]] |
[[Category: Schneemann, A.]] | [[Category: Schneemann, A.]] | ||
| - | [[Category: Williamson, J | + | [[Category: Williamson, J R.]] |
[[Category: four-helix bundle]] | [[Category: four-helix bundle]] | ||
[[Category: protein-rna complex]] | [[Category: protein-rna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:32:33 2008'' |
Revision as of 14:32, 21 February 2008
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Flock House virus B2-dsRNA Complex (P4122)
Overview
As a counter-defense against antiviral RNA silencing during infection, the insect Flock House virus (FHV) expresses the silencing suppressor protein B2. Biochemical experiments show that B2 binds to double-stranded RNA (dsRNA) without regard to length and inhibits cleavage of dsRNA by Dicer in vitro. A cocrystal structure reveals that a B2 dimer forms a four-helix bundle that binds to one face of an A-form RNA duplex independently of sequence. These results suggest that B2 blocks both cleavage of the FHV genome by Dicer and incorporation of FHV small interfering RNAs into the RNA-induced silencing complex.
About this Structure
2AZ2 is a Single protein structure of sequence from Flock house virus. Full crystallographic information is available from OCA.
Reference
Dual modes of RNA-silencing suppression by Flock House virus protein B2., Chao JA, Lee JH, Chapados BR, Debler EW, Schneemann A, Williamson JR, Nat Struct Mol Biol. 2005 Nov;12(11):952-7. PMID:16228003
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