2b1u
From Proteopedia
(New page: 200px<br /> <applet load="2b1u" size="450" color="white" frame="true" align="right" spinBox="true" caption="2b1u" /> '''Solution structure of Calmodulin-like Skin ...) |
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'''Solution structure of Calmodulin-like Skin Protein C terminal domain'''<br /> | '''Solution structure of Calmodulin-like Skin Protein C terminal domain'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure and dynamics of human calmodulin-like skin protein (CLSP) | + | The structure and dynamics of human calmodulin-like skin protein (CLSP) have been characterized by NMR spectroscopy. The mobility of CLSP has been found to be different for the N-terminal and C-terminal domains. The isolated domains were also expressed and analyzed. The structure of the isolated C-terminal domain is presented. The N-terminal domain is characterized by four stable helices, which experience large fluctuations. This is shown to be due to mutations in the hydrophobic core. The overall N-terminal domain behavior is similar both in the full-length protein and in the isolated domain. By exploiting the capability of Tb3+ bound to CLSP to induce partial orientation of the molecule in a magnetic field, restricted motion of one domain with respect to the other was proved. By using NMR, ITC, and ESI-MS, the calcium and magnesium binding properties were investigated. Finally, CLSP is framed into the evolutionary scheme of the calmodulin-like family. |
==About this Structure== | ==About this Structure== | ||
| - | 2B1U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 2B1U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B1U OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Chirivino, E.]] | [[Category: Chirivino, E.]] | ||
[[Category: Luchinat, C.]] | [[Category: Luchinat, C.]] | ||
| - | [[Category: SPINE, Structural | + | [[Category: SPINE, Structural Proteomics in Europe.]] |
[[Category: backbone dynamic]] | [[Category: backbone dynamic]] | ||
[[Category: calmodulin-like skin protein]] | [[Category: calmodulin-like skin protein]] | ||
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[[Category: structural proteomics in europe]] | [[Category: structural proteomics in europe]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:33:22 2008'' |
Revision as of 14:33, 21 February 2008
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Solution structure of Calmodulin-like Skin Protein C terminal domain
Overview
The structure and dynamics of human calmodulin-like skin protein (CLSP) have been characterized by NMR spectroscopy. The mobility of CLSP has been found to be different for the N-terminal and C-terminal domains. The isolated domains were also expressed and analyzed. The structure of the isolated C-terminal domain is presented. The N-terminal domain is characterized by four stable helices, which experience large fluctuations. This is shown to be due to mutations in the hydrophobic core. The overall N-terminal domain behavior is similar both in the full-length protein and in the isolated domain. By exploiting the capability of Tb3+ bound to CLSP to induce partial orientation of the molecule in a magnetic field, restricted motion of one domain with respect to the other was proved. By using NMR, ITC, and ESI-MS, the calcium and magnesium binding properties were investigated. Finally, CLSP is framed into the evolutionary scheme of the calmodulin-like family.
About this Structure
2B1U is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
A structural and dynamic characterization of the EF-hand protein CLSP., Babini E, Bertini I, Capozzi F, Chirivino E, Luchinat C, Structure. 2006 Jun;14(6):1029-38. PMID:16765896
Page seeded by OCA on Thu Feb 21 16:33:22 2008
Categories: Homo sapiens | Single protein | Babini, E. | Bertini, I. | Capozzi, F. | Chirivino, E. | Luchinat, C. | SPINE, Structural Proteomics in Europe. | Backbone dynamic | Calmodulin-like skin protein | Clsp | Nmr | Solution structure | Spine | Structural genomics | Structural proteomics in europe
