2b5p
From Proteopedia
(New page: 200px<br /><applet load="2b5p" size="450" color="white" frame="true" align="right" spinBox="true" caption="2b5p" /> '''Solution structure of ribbon isoform of CMrV...) |
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'''Solution structure of ribbon isoform of CMrVIA lambda conotoxin'''<br /> | '''Solution structure of ribbon isoform of CMrVIA lambda conotoxin'''<br /> | ||
==Overview== | ==Overview== | ||
| - | alpha-Conotoxins possess a conserved four-cysteine framework and disulfide | + | alpha-Conotoxins possess a conserved four-cysteine framework and disulfide linkages (C(1)(-)(3), C(2)(-)(4)) that fold toward the globular conformation with absolute fidelity. Despite the presence of a similar conserved set of cysteine framework, chi/lambda-conotoxins adopt an alternate disulfide-pairing (C(1)(-)(4), C(2)(-)(3)) and its consequent ribbon conformation, exhibiting distinct biological activities from alpha-conotoxins. chi/lambda-Conotoxin CMrVIA (VCCGYKLCHOC-COOH) isolated from the venom of Conus marmoreus natively exists in the ribbon conformation and induces seizures in mice at a potency that is of three orders higher than the non-native globular form. We have chemically synthesized two isoforms of CMrVIA conotoxin in the ribbon and globular conformation and determined their structures by (1)H NMR spectroscopy. The ribbon (PDB ID 2B5P) and globular conformations (PBD ID 2B5Q) were calculated to have paired-wise backbone RMSDs of 0.48 +/- 0.1 and 0.58 +/- 0.1 A respectively. Unlike the native globular alpha-conotoxins, the globular canonical form of CMrVIA chi/lambda-conotoxin exhibited heterogeneity in its solution structure as noted by the presence of minor conformers and poorer RMSD of structure calculation. Paired-wise backbone comparison between the native ribbon and the non-native globular form of CMrVIA conotoxin revealed an RMSD of 4.73 A, emphasizing their distinct conformational differences. These structural data are essential for the understanding of the structure-function activity of chi/lambda-conotoxins, as well as unraveling the folding propensities of these short peptide toxins. |
==About this Structure== | ==About this Structure== | ||
| - | 2B5P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 2B5P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5P OCA]. |
==Reference== | ==Reference== | ||
Solution structures of two structural isoforms of CMrVIA chi/lambda-conotoxin., Kang TS, Jois SD, Kini RM, Biomacromolecules. 2006 Aug;7(8):2337-46. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16903680 16903680] | Solution structures of two structural isoforms of CMrVIA chi/lambda-conotoxin., Kang TS, Jois SD, Kini RM, Biomacromolecules. 2006 Aug;7(8):2337-46. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16903680 16903680] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Jois, S | + | [[Category: Jois, S D.S.]] |
| - | [[Category: Kang, T | + | [[Category: Kang, T S.]] |
| - | [[Category: Kini, R | + | [[Category: Kini, R M.]] |
[[Category: conotoxin]] | [[Category: conotoxin]] | ||
[[Category: disulfide linkage]] | [[Category: disulfide linkage]] | ||
[[Category: ribbon conformation]] | [[Category: ribbon conformation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:34:27 2008'' |
Revision as of 14:34, 21 February 2008
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Solution structure of ribbon isoform of CMrVIA lambda conotoxin
Overview
alpha-Conotoxins possess a conserved four-cysteine framework and disulfide linkages (C(1)(-)(3), C(2)(-)(4)) that fold toward the globular conformation with absolute fidelity. Despite the presence of a similar conserved set of cysteine framework, chi/lambda-conotoxins adopt an alternate disulfide-pairing (C(1)(-)(4), C(2)(-)(3)) and its consequent ribbon conformation, exhibiting distinct biological activities from alpha-conotoxins. chi/lambda-Conotoxin CMrVIA (VCCGYKLCHOC-COOH) isolated from the venom of Conus marmoreus natively exists in the ribbon conformation and induces seizures in mice at a potency that is of three orders higher than the non-native globular form. We have chemically synthesized two isoforms of CMrVIA conotoxin in the ribbon and globular conformation and determined their structures by (1)H NMR spectroscopy. The ribbon (PDB ID 2B5P) and globular conformations (PBD ID 2B5Q) were calculated to have paired-wise backbone RMSDs of 0.48 +/- 0.1 and 0.58 +/- 0.1 A respectively. Unlike the native globular alpha-conotoxins, the globular canonical form of CMrVIA chi/lambda-conotoxin exhibited heterogeneity in its solution structure as noted by the presence of minor conformers and poorer RMSD of structure calculation. Paired-wise backbone comparison between the native ribbon and the non-native globular form of CMrVIA conotoxin revealed an RMSD of 4.73 A, emphasizing their distinct conformational differences. These structural data are essential for the understanding of the structure-function activity of chi/lambda-conotoxins, as well as unraveling the folding propensities of these short peptide toxins.
About this Structure
2B5P is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Solution structures of two structural isoforms of CMrVIA chi/lambda-conotoxin., Kang TS, Jois SD, Kini RM, Biomacromolecules. 2006 Aug;7(8):2337-46. PMID:16903680
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