2b63
From Proteopedia
(New page: 200px<br /><applet load="2b63" size="450" color="white" frame="true" align="right" spinBox="true" caption="2b63, resolution 3.800Å" /> '''Complete RNA Polyme...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2b63.gif|left|200px]]<br /><applet load="2b63" size=" | + | [[Image:2b63.gif|left|200px]]<br /><applet load="2b63" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2b63, resolution 3.800Å" /> | caption="2b63, resolution 3.800Å" /> | ||
'''Complete RNA Polymerase II-RNA inhibitor complex'''<br /> | '''Complete RNA Polymerase II-RNA inhibitor complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The noncoding RNA B2 and the RNA aptamer FC bind RNA polymerase (Pol) II | + | The noncoding RNA B2 and the RNA aptamer FC bind RNA polymerase (Pol) II and inhibit messenger RNA transcription initiation, but not elongation. We report the crystal structure of FC(*), the central part of FC RNA, bound to Pol II. FC(*) RNA forms a double stem-loop structure in the Pol II active center cleft. B2 RNA may bind similarly, as it competes with FC(*) RNA for Pol II interaction. Both RNA inhibitors apparently prevent the downstream DNA duplex and the template single strand from entering the cleft after DNA melting and thus interfere with open-complex formation. Elongation is not inhibited, as nucleic acids prebound in the cleft would exclude the RNA inhibitors. The structure also indicates that A-form RNA could interact with Pol II similarly to a B-form DNA promoter, as suggested for the bacterial transcription inhibitor 6S RNA. |
==About this Structure== | ==About this Structure== | ||
| - | 2B63 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ZN and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http:// | + | 2B63 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B63 OCA]. |
==Reference== | ==Reference== | ||
| Line 28: | Line 28: | ||
[[Category: rna polymerase ii]] | [[Category: rna polymerase ii]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:34:38 2008'' |
Revision as of 14:34, 21 February 2008
|
Complete RNA Polymerase II-RNA inhibitor complex
Overview
The noncoding RNA B2 and the RNA aptamer FC bind RNA polymerase (Pol) II and inhibit messenger RNA transcription initiation, but not elongation. We report the crystal structure of FC(*), the central part of FC RNA, bound to Pol II. FC(*) RNA forms a double stem-loop structure in the Pol II active center cleft. B2 RNA may bind similarly, as it competes with FC(*) RNA for Pol II interaction. Both RNA inhibitors apparently prevent the downstream DNA duplex and the template single strand from entering the cleft after DNA melting and thus interfere with open-complex formation. Elongation is not inhibited, as nucleic acids prebound in the cleft would exclude the RNA inhibitors. The structure also indicates that A-form RNA could interact with Pol II similarly to a B-form DNA promoter, as suggested for the bacterial transcription inhibitor 6S RNA.
About this Structure
2B63 is a Protein complex structure of sequences from Saccharomyces cerevisiae with and as ligands. Active as DNA-directed RNA polymerase, with EC number 2.7.7.6 Full crystallographic information is available from OCA.
Reference
Structure of an RNA polymerase II-RNA inhibitor complex elucidates transcription regulation by noncoding RNAs., Kettenberger H, Eisenfuhr A, Brueckner F, Theis M, Famulok M, Cramer P, Nat Struct Mol Biol. 2006 Jan;13(1):44-8. Epub 2005 Dec 11. PMID:16341226
Page seeded by OCA on Thu Feb 21 16:34:38 2008
