2b7y
From Proteopedia
(New page: 200px<br /><applet load="2b7y" size="450" color="white" frame="true" align="right" spinBox="true" caption="2b7y, resolution 3.00Å" /> '''Fava Bean Lectin-Glu...) |
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| - | [[Image:2b7y.gif|left|200px]]<br /><applet load="2b7y" size=" | + | [[Image:2b7y.gif|left|200px]]<br /><applet load="2b7y" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2b7y, resolution 3.00Å" /> | caption="2b7y, resolution 3.00Å" /> | ||
'''Fava Bean Lectin-Glucose Complex'''<br /> | '''Fava Bean Lectin-Glucose Complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The three-dimensional structure of favin, the glucose- and mannose-binding | + | The three-dimensional structure of favin, the glucose- and mannose-binding lectin of Vicia faba (vetch, broad bean), has been determined at a resolution of 2.8 angstroms by molecular replacement. The crystals contain specifically bound glucose and provide the first high-resolution view of specific saccharide binding in a leguminous lectin. The structure is similar to those of concanavalin A (Con A) and green pea lectin; differences from Con A show that minimal changes are needed to accommodate the cyclic permutation in amino acid sequence between the two molecules. The molecule is an ellipsoidal dimer dominated by extensive beta structures. Each protomer contains binding sites for two divalent metal ions (Mn2+ and Ca2+) and a specific saccharide. Glucose is bound by favin in a cleft in the molecular surface and has noncovalent contacts primarily with two peptide loops, one of which contains several metal ion ligands. The specific carbohydrate-binding site is similar to that of Con A in location and general peptide folding, despite several differences in specific amino acid residues. |
==About this Structure== | ==About this Structure== | ||
| - | 2B7Y is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Vicia_faba Vicia faba] with GLC, NDG, MN and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2B7Y is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Vicia_faba Vicia faba] with <scene name='pdbligand=GLC:'>GLC</scene>, <scene name='pdbligand=NDG:'>NDG</scene>, <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B7Y OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Vicia faba]] | [[Category: Vicia faba]] | ||
| - | [[Category: Becker, J | + | [[Category: Becker, J W.]] |
| - | [[Category: Jr., G | + | [[Category: Jr., G N.Reeke.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: GLC]] | [[Category: GLC]] | ||
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[[Category: protein-carbohydrate complex]] | [[Category: protein-carbohydrate complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:35:12 2008'' |
Revision as of 14:35, 21 February 2008
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Fava Bean Lectin-Glucose Complex
Overview
The three-dimensional structure of favin, the glucose- and mannose-binding lectin of Vicia faba (vetch, broad bean), has been determined at a resolution of 2.8 angstroms by molecular replacement. The crystals contain specifically bound glucose and provide the first high-resolution view of specific saccharide binding in a leguminous lectin. The structure is similar to those of concanavalin A (Con A) and green pea lectin; differences from Con A show that minimal changes are needed to accommodate the cyclic permutation in amino acid sequence between the two molecules. The molecule is an ellipsoidal dimer dominated by extensive beta structures. Each protomer contains binding sites for two divalent metal ions (Mn2+ and Ca2+) and a specific saccharide. Glucose is bound by favin in a cleft in the molecular surface and has noncovalent contacts primarily with two peptide loops, one of which contains several metal ion ligands. The specific carbohydrate-binding site is similar to that of Con A in location and general peptide folding, despite several differences in specific amino acid residues.
About this Structure
2B7Y is a Protein complex structure of sequences from Vicia faba with , , and as ligands. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of favin: saccharide binding-cyclic permutation in leguminous lectins., Reeke GN Jr, Becker JW, Science. 1986 Nov 28;234(4780):1108-11. PMID:3775378
Page seeded by OCA on Thu Feb 21 16:35:12 2008
