2b9n
From Proteopedia
(New page: 200px<br /><applet load="2b9n" size="450" color="white" frame="true" align="right" spinBox="true" caption="2b9n, resolution 6.76Å" /> '''50S ribosomal subuni...) |
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| - | [[Image:2b9n.gif|left|200px]]<br /><applet load="2b9n" size=" | + | [[Image:2b9n.gif|left|200px]]<br /><applet load="2b9n" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2b9n, resolution 6.76Å" /> | caption="2b9n, resolution 6.76Å" /> | ||
'''50S ribosomal subunit from a crystal structure of release factor RF2, tRNAs and mRNA bound to the ribosome. This file contains the 50S subunit from a crystal structure of release factor RF1, tRNAs and mRNA bound to the ribosome and is described in remark 400.'''<br /> | '''50S ribosomal subunit from a crystal structure of release factor RF2, tRNAs and mRNA bound to the ribosome. This file contains the 50S subunit from a crystal structure of release factor RF1, tRNAs and mRNA bound to the ribosome and is described in remark 400.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | During protein synthesis, translational release factors catalyze the | + | During protein synthesis, translational release factors catalyze the release of the polypeptide chain when a stop codon on the mRNA reaches the A site of the ribosome. The detailed mechanism of this process is currently unknown. We present here the crystal structures of the ribosome from Thermus thermophilus with RF1 and RF2 bound to their cognate stop codons, at resolutions of 5.9 Angstrom and 6.7 Angstrom, respectively. The structures reveal details of interactions of the factors with the ribosome and mRNA, including elements previously implicated in decoding and peptide release. They also shed light on conformational changes both in the factors and in the ribosome during termination. Differences seen in the interaction of RF1 and RF2 with the L11 region of the ribosome allow us to rationalize previous biochemical data. Finally, this work demonstrates the feasibility of crystallizing ribosomes with bound factors at a defined state along the translational pathway. |
==About this Structure== | ==About this Structure== | ||
| - | 2B9N is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http:// | + | 2B9N is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B9N OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
| - | [[Category: Brodersen, D | + | [[Category: Brodersen, D E.]] |
| - | [[Category: Dunham, C | + | [[Category: Dunham, C M.]] |
| - | [[Category: IV, F | + | [[Category: IV, F V.Murphy.]] |
| - | [[Category: Kelley, A | + | [[Category: Kelley, A C.]] |
[[Category: Petry, S.]] | [[Category: Petry, S.]] | ||
[[Category: Ramakrishnan, V.]] | [[Category: Ramakrishnan, V.]] | ||
[[Category: Selmer, M.]] | [[Category: Selmer, M.]] | ||
| - | [[Category: Tarry, M | + | [[Category: Tarry, M J.]] |
[[Category: release factor]] | [[Category: release factor]] | ||
[[Category: ribosome]] | [[Category: ribosome]] | ||
[[Category: translation]] | [[Category: translation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:35:35 2008'' |
Revision as of 14:35, 21 February 2008
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50S ribosomal subunit from a crystal structure of release factor RF2, tRNAs and mRNA bound to the ribosome. This file contains the 50S subunit from a crystal structure of release factor RF1, tRNAs and mRNA bound to the ribosome and is described in remark 400.
Overview
During protein synthesis, translational release factors catalyze the release of the polypeptide chain when a stop codon on the mRNA reaches the A site of the ribosome. The detailed mechanism of this process is currently unknown. We present here the crystal structures of the ribosome from Thermus thermophilus with RF1 and RF2 bound to their cognate stop codons, at resolutions of 5.9 Angstrom and 6.7 Angstrom, respectively. The structures reveal details of interactions of the factors with the ribosome and mRNA, including elements previously implicated in decoding and peptide release. They also shed light on conformational changes both in the factors and in the ribosome during termination. Differences seen in the interaction of RF1 and RF2 with the L11 region of the ribosome allow us to rationalize previous biochemical data. Finally, this work demonstrates the feasibility of crystallizing ribosomes with bound factors at a defined state along the translational pathway.
About this Structure
2B9N is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
Crystal structures of the ribosome in complex with release factors RF1 and RF2 bound to a cognate stop codon., Petry S, Brodersen DE, Murphy FV 4th, Dunham CM, Selmer M, Tarry MJ, Kelley AC, Ramakrishnan V, Cell. 2005 Dec 29;123(7):1255-66. PMID:16377566
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