2bab
From Proteopedia
(New page: 200px<br /><applet load="2bab" size="350" color="white" frame="true" align="right" spinBox="true" caption="2bab, resolution 2.00Å" /> '''Crystal structure of...) |
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==Overview== | ==Overview== | ||
| - | Conjugated linoleic acids (CLAs) affect body fat gain, carcinogenesis, insulin resistance, and lipid peroxidation in mammals. Several isomers of | + | Conjugated linoleic acids (CLAs) affect body fat gain, carcinogenesis, insulin resistance, and lipid peroxidation in mammals. Several isomers of CLA exist, of which the (9Z, 11E) and (10E, 12Z) isomers have beneficial effects on human metabolism but are scarce in foods. Bacterial polyunsaturated fatty acid isomerases are promising biotechnological catalysts for CLA production. We describe six crystal structures of the Propionibacterium acnes polyunsaturated fatty acid isomerase PAI in apo- and product-bound forms. The three-domain flavoprotein has previously undescribed folds outside the FAD-binding site. Conformational changes in a hydrophobic channel toward the active site reveal a unique gating mechanism for substrate specificity. The geometry of the substrate-binding site explains the length preferences for C18 fatty acids. A catalytic mechanism for double-bond isomerization is formulated that may be altered to change substrate specificity for syntheses of rare CLAs from easily accessible precursors. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Liavonchanka, A.]] | [[Category: Liavonchanka, A.]] | ||
| - | [[Category: Rudolph, M | + | [[Category: Rudolph, M G.]] |
[[Category: BU1]] | [[Category: BU1]] | ||
[[Category: FAD]] | [[Category: FAD]] | ||
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[[Category: isomerase]] | [[Category: isomerase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:35:40 2008'' |
Revision as of 14:35, 21 February 2008
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Crystal structure of CLA-producing fatty acid isomerase from P. acnes
Overview
Conjugated linoleic acids (CLAs) affect body fat gain, carcinogenesis, insulin resistance, and lipid peroxidation in mammals. Several isomers of CLA exist, of which the (9Z, 11E) and (10E, 12Z) isomers have beneficial effects on human metabolism but are scarce in foods. Bacterial polyunsaturated fatty acid isomerases are promising biotechnological catalysts for CLA production. We describe six crystal structures of the Propionibacterium acnes polyunsaturated fatty acid isomerase PAI in apo- and product-bound forms. The three-domain flavoprotein has previously undescribed folds outside the FAD-binding site. Conformational changes in a hydrophobic channel toward the active site reveal a unique gating mechanism for substrate specificity. The geometry of the substrate-binding site explains the length preferences for C18 fatty acids. A catalytic mechanism for double-bond isomerization is formulated that may be altered to change substrate specificity for syntheses of rare CLAs from easily accessible precursors.
About this Structure
2BAB is a Single protein structure of sequence from Propionibacterium acnes with , , and as ligands. Full crystallographic information is available from OCA.
Reference
Structure and mechanism of the Propionibacterium acnes polyunsaturated fatty acid isomerase., Liavonchanka A, Hornung E, Feussner I, Rudolph MG, Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2576-81. Epub 2006 Feb 13. PMID:16477020
Page seeded by OCA on Thu Feb 21 16:35:40 2008
Categories: Propionibacterium acnes | Single protein | Liavonchanka, A. | Rudolph, M G. | BU1 | FAD | ODD | SO4 | Conjugated linoleic acid | Fad | Isomerase
