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2bay
From Proteopedia
(New page: 200px<br /><applet load="2bay" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bay, resolution 1.50Å" /> '''Crystal structure of...) |
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| - | [[Image:2bay.gif|left|200px]]<br /><applet load="2bay" size=" | + | [[Image:2bay.gif|left|200px]]<br /><applet load="2bay" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2bay, resolution 1.50Å" /> | caption="2bay, resolution 1.50Å" /> | ||
'''Crystal structure of the Prp19 U-box dimer'''<br /> | '''Crystal structure of the Prp19 U-box dimer'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Prp19 is an essential splicing factor and a member of the U-box family of | + | Prp19 is an essential splicing factor and a member of the U-box family of E3 ubiquitin ligases. Prp19 forms a tetramer via a central coiled-coil domain. Here, we show the U-box domain of Prp19 exists as a dimer within the context of the Prp19 tetramer. A high-resolution structure of the homodimeric state of the Prp19 U-box was determined by X-ray crystallography. Mutation of the U-box dimer interface abrogates U-box dimer formation and is lethal in vivo. The structure of the U-box dimer enables construction of a complete model of Prp19 providing insights into how the tetrameric protein functions as an E3 ligase. Finally, comparison of the Prp19 U-box homodimer with the heterodimeric complex of BRCA1/BARD1 RING-finger domains uncovers a common architecture for a family of oligomeric U-box and RING-finger E3 ubiquitin ligases, which has mechanistic implications for E3 ligase-mediated polyubiquitination and E4 polyubiquitin ligases. |
==About this Structure== | ==About this Structure== | ||
| - | 2BAY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http:// | + | 2BAY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BAY OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chazin, W | + | [[Category: Chazin, W J.]] |
| - | [[Category: Gould, K | + | [[Category: Gould, K L.]] |
| - | [[Category: Kooi, C | + | [[Category: Kooi, C W.Vander.]] |
| - | [[Category: Newcomer, M | + | [[Category: Newcomer, M E.]] |
| - | [[Category: Ohi, M | + | [[Category: Ohi, M D.]] |
| - | [[Category: Oldham, M | + | [[Category: Oldham, M L.]] |
| - | [[Category: Rosenberg, J | + | [[Category: Rosenberg, J A.]] |
[[Category: e3 ligase]] | [[Category: e3 ligase]] | ||
[[Category: prp19]] | [[Category: prp19]] | ||
| Line 25: | Line 25: | ||
[[Category: ubiquitin ligase]] | [[Category: ubiquitin ligase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:35:50 2008'' |
Revision as of 14:35, 21 February 2008
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Crystal structure of the Prp19 U-box dimer
Overview
Prp19 is an essential splicing factor and a member of the U-box family of E3 ubiquitin ligases. Prp19 forms a tetramer via a central coiled-coil domain. Here, we show the U-box domain of Prp19 exists as a dimer within the context of the Prp19 tetramer. A high-resolution structure of the homodimeric state of the Prp19 U-box was determined by X-ray crystallography. Mutation of the U-box dimer interface abrogates U-box dimer formation and is lethal in vivo. The structure of the U-box dimer enables construction of a complete model of Prp19 providing insights into how the tetrameric protein functions as an E3 ligase. Finally, comparison of the Prp19 U-box homodimer with the heterodimeric complex of BRCA1/BARD1 RING-finger domains uncovers a common architecture for a family of oligomeric U-box and RING-finger E3 ubiquitin ligases, which has mechanistic implications for E3 ligase-mediated polyubiquitination and E4 polyubiquitin ligases.
About this Structure
2BAY is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
The Prp19 U-box crystal structure suggests a common dimeric architecture for a class of oligomeric E3 ubiquitin ligases., Vander Kooi CW, Ohi MD, Rosenberg JA, Oldham ML, Newcomer ME, Gould KL, Chazin WJ, Biochemistry. 2006 Jan 10;45(1):121-30. PMID:16388587
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