2nun
From Proteopedia
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{{STRUCTURE_2nun| PDB=2nun | SCENE= }} | {{STRUCTURE_2nun| PDB=2nun | SCENE= }} | ||
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===The structure of the type III effector AvrB complexed with ADP=== | ===The structure of the type III effector AvrB complexed with ADP=== | ||
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==See Also== | ==See Also== | ||
| + | *[[Avirulence protein|Avirulence protein]] | ||
*[[Type III effector AvrB complexed with ADP|Type III effector AvrB complexed with ADP]] | *[[Type III effector AvrB complexed with ADP|Type III effector AvrB complexed with ADP]] | ||
Revision as of 10:10, 11 March 2013
Contents |
The structure of the type III effector AvrB complexed with ADP
The Pseudomonas syringae type III effector protein avirulence protein B (AvrB) is delivered into plant cells, where it targets the Arabidopsis RIN4 protein (resistance to Pseudomonas maculicula protein 1 [RPM1]-interacting protein). RIN4 is a regulator of basal host defense responses. Targeting of RIN4 by AvrB is recognized by the host RPM1 nucleotide-binding leucine-rich repeat disease resistance protein, leading to accelerated defense responses, cessation of pathogen growth, and hypersensitive host cell death at the infection site. We determined the structure of AvrB complexed with an AvrB-binding fragment of RIN4 at 2.3 A resolution. We also determined the structure of AvrB in complex with adenosine diphosphate bound in a binding pocket adjacent to the RIN4 binding domain. AvrB residues important for RIN4 interaction are required for full RPM1 activation. AvrB residues that contact adenosine diphosphate are also required for initiation of RPM1 function. Nucleotide-binding residues of AvrB are also required for its phosphorylation by an unknown Arabidopsis protein(s). We conclude that AvrB is activated inside the host cell by nucleotide binding and subsequent phosphorylation and, independently, interacts with RIN4. Our data suggest that activated AvrB, bound to RIN4, is indirectly recognized by RPM1 to initiate plant immune system function.
Type III effector activation via nucleotide binding, phosphorylation, and host target interaction., Desveaux D, Singer AU, Wu AJ, McNulty BC, Musselwhite L, Nimchuk Z, Sondek J, Dangl JL, PLoS Pathog. 2007 Mar;3(3):e48. PMID:17397263
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2nun is a 1 chain structure with sequence from Pseudomonas syringae pv. glycinea. Full crystallographic information is available from OCA.
See Also
Reference
- Desveaux D, Singer AU, Wu AJ, McNulty BC, Musselwhite L, Nimchuk Z, Sondek J, Dangl JL. Type III effector activation via nucleotide binding, phosphorylation, and host target interaction. PLoS Pathog. 2007 Mar;3(3):e48. PMID:17397263 doi:10.1371/journal.ppat.0030048
