2bep
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Post-translational modification with small ubiquitin-related modifier | + | Post-translational modification with small ubiquitin-related modifier (SUMO) alters the function of many proteins, but the molecular mechanisms and consequences of this modification are still poorly defined. During a screen for novel SUMO1 targets, we identified the ubiquitin-conjugating enzyme E2-25K (Hip2). SUMO attachment severely impairs E2-25K ubiquitin thioester and unanchored ubiquitin chain formation in vitro. Crystal structures of E2-25K(1-155) and of the E2-25K(1-155)-SUMO conjugate (E2-25K(*)SUMO) indicate that SUMO attachment interferes with E1 interaction through its location on the N-terminal helix. The SUMO acceptor site in E2-25K, Lys14, does not conform to the consensus site found in most SUMO targets (PsiKXE), and functions only in the context of an alpha-helix. In contrast, adjacent SUMO consensus sites are modified only when in unstructured peptides. The demonstration that secondary structure elements are part of SUMO attachment signals could contribute to a better prediction of SUMO targets. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Ubiquitin--protein ligase]] | [[Category: Ubiquitin--protein ligase]] | ||
| - | [[Category: Dijk, W | + | [[Category: Dijk, W J.Van.]] |
[[Category: Jentsch, S.]] | [[Category: Jentsch, S.]] | ||
[[Category: Knipscheer, P.]] | [[Category: Knipscheer, P.]] | ||
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[[Category: Melchior, F.]] | [[Category: Melchior, F.]] | ||
[[Category: Oberhofer, E.]] | [[Category: Oberhofer, E.]] | ||
| - | [[Category: Olsen, J | + | [[Category: Olsen, J Velgaard.]] |
[[Category: Pichler, A.]] | [[Category: Pichler, A.]] | ||
| - | [[Category: SPINE, Structural | + | [[Category: SPINE, Structural Proteomics in Europe.]] |
| - | [[Category: Sixma, T | + | [[Category: Sixma, T K.]] |
[[Category: BME]] | [[Category: BME]] | ||
[[Category: e2 conjugating enzyme]] | [[Category: e2 conjugating enzyme]] | ||
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[[Category: ubiquitin]] | [[Category: ubiquitin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:37:02 2008'' |
Revision as of 14:37, 21 February 2008
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CRYSTAL STRUCTURE OF UBIQUITIN CONJUGATING ENZYME E2-25K
Overview
Post-translational modification with small ubiquitin-related modifier (SUMO) alters the function of many proteins, but the molecular mechanisms and consequences of this modification are still poorly defined. During a screen for novel SUMO1 targets, we identified the ubiquitin-conjugating enzyme E2-25K (Hip2). SUMO attachment severely impairs E2-25K ubiquitin thioester and unanchored ubiquitin chain formation in vitro. Crystal structures of E2-25K(1-155) and of the E2-25K(1-155)-SUMO conjugate (E2-25K(*)SUMO) indicate that SUMO attachment interferes with E1 interaction through its location on the N-terminal helix. The SUMO acceptor site in E2-25K, Lys14, does not conform to the consensus site found in most SUMO targets (PsiKXE), and functions only in the context of an alpha-helix. In contrast, adjacent SUMO consensus sites are modified only when in unstructured peptides. The demonstration that secondary structure elements are part of SUMO attachment signals could contribute to a better prediction of SUMO targets.
About this Structure
2BEP is a Single protein structure of sequence from Bos taurus with as ligand. Active as Ubiquitin--protein ligase, with EC number 6.3.2.19 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
SUMO modification of the ubiquitin-conjugating enzyme E2-25K., Pichler A, Knipscheer P, Oberhofer E, van Dijk WJ, Korner R, Olsen JV, Jentsch S, Melchior F, Sixma TK, Nat Struct Mol Biol. 2005 Mar;12(3):264-9. Epub 2005 Feb 20. PMID:15723079
Page seeded by OCA on Thu Feb 21 16:37:02 2008
Categories: Bos taurus | Single protein | Ubiquitin--protein ligase | Dijk, W J.Van. | Jentsch, S. | Knipscheer, P. | Korner, R. | Melchior, F. | Oberhofer, E. | Olsen, J Velgaard. | Pichler, A. | SPINE, Structural Proteomics in Europe. | Sixma, T K. | BME | E2 conjugating enzyme | Ligase | Protein degradation | Spine | Structural genomics | Structural proteomics in europe | Ubiquitin
