2bf8
From Proteopedia
(New page: 200px<br /> <applet load="2bf8" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bf8, resolution 2.30Å" /> '''CRYSTAL STRUCTURE O...) |
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| - | [[Image:2bf8.gif|left|200px]]<br /> | + | [[Image:2bf8.gif|left|200px]]<br /><applet load="2bf8" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="2bf8, resolution 2.30Å" /> | caption="2bf8, resolution 2.30Å" /> | ||
'''CRYSTAL STRUCTURE OF SUMO MODIFIED UBIQUITIN CONJUGATING ENZYME E2-25K'''<br /> | '''CRYSTAL STRUCTURE OF SUMO MODIFIED UBIQUITIN CONJUGATING ENZYME E2-25K'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Post-translational modification with small ubiquitin-related modifier | + | Post-translational modification with small ubiquitin-related modifier (SUMO) alters the function of many proteins, but the molecular mechanisms and consequences of this modification are still poorly defined. During a screen for novel SUMO1 targets, we identified the ubiquitin-conjugating enzyme E2-25K (Hip2). SUMO attachment severely impairs E2-25K ubiquitin thioester and unanchored ubiquitin chain formation in vitro. Crystal structures of E2-25K(1-155) and of the E2-25K(1-155)-SUMO conjugate (E2-25K(*)SUMO) indicate that SUMO attachment interferes with E1 interaction through its location on the N-terminal helix. The SUMO acceptor site in E2-25K, Lys14, does not conform to the consensus site found in most SUMO targets (PsiKXE), and functions only in the context of an alpha-helix. In contrast, adjacent SUMO consensus sites are modified only when in unstructured peptides. The demonstration that secondary structure elements are part of SUMO attachment signals could contribute to a better prediction of SUMO targets. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2BF8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] Full crystallographic information is available from [http:// | + | 2BF8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BF8 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Ubiquitin--protein ligase]] | [[Category: Ubiquitin--protein ligase]] | ||
| - | [[Category: Dijk, W | + | [[Category: Dijk, W J.Van.]] |
[[Category: Jentsch, S.]] | [[Category: Jentsch, S.]] | ||
[[Category: Knipscheer, P.]] | [[Category: Knipscheer, P.]] | ||
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[[Category: Melchior, F.]] | [[Category: Melchior, F.]] | ||
[[Category: Oberhofer, E.]] | [[Category: Oberhofer, E.]] | ||
| - | [[Category: Olsen, J | + | [[Category: Olsen, J Velgaard.]] |
[[Category: Pichler, A.]] | [[Category: Pichler, A.]] | ||
| - | [[Category: SPINE, Structural | + | [[Category: SPINE, Structural Proteomics in Europe.]] |
| - | [[Category: Sixma, T | + | [[Category: Sixma, T K.]] |
[[Category: e2 ubiquitin conjugating enzyme]] | [[Category: e2 ubiquitin conjugating enzyme]] | ||
[[Category: e2-25k]] | [[Category: e2-25k]] | ||
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[[Category: sumo-target structure]] | [[Category: sumo-target structure]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:37:12 2008'' |
Revision as of 14:37, 21 February 2008
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CRYSTAL STRUCTURE OF SUMO MODIFIED UBIQUITIN CONJUGATING ENZYME E2-25K
Contents |
Overview
Post-translational modification with small ubiquitin-related modifier (SUMO) alters the function of many proteins, but the molecular mechanisms and consequences of this modification are still poorly defined. During a screen for novel SUMO1 targets, we identified the ubiquitin-conjugating enzyme E2-25K (Hip2). SUMO attachment severely impairs E2-25K ubiquitin thioester and unanchored ubiquitin chain formation in vitro. Crystal structures of E2-25K(1-155) and of the E2-25K(1-155)-SUMO conjugate (E2-25K(*)SUMO) indicate that SUMO attachment interferes with E1 interaction through its location on the N-terminal helix. The SUMO acceptor site in E2-25K, Lys14, does not conform to the consensus site found in most SUMO targets (PsiKXE), and functions only in the context of an alpha-helix. In contrast, adjacent SUMO consensus sites are modified only when in unstructured peptides. The demonstration that secondary structure elements are part of SUMO attachment signals could contribute to a better prediction of SUMO targets.
Disease
Known diseases associated with this structure: Orofacial cleft 10 OMIM:[601912]
About this Structure
2BF8 is a Protein complex structure of sequences from Bos taurus and Homo sapiens. Active as Ubiquitin--protein ligase, with EC number 6.3.2.19 Full crystallographic information is available from OCA.
Reference
SUMO modification of the ubiquitin-conjugating enzyme E2-25K., Pichler A, Knipscheer P, Oberhofer E, van Dijk WJ, Korner R, Olsen JV, Jentsch S, Melchior F, Sixma TK, Nat Struct Mol Biol. 2005 Mar;12(3):264-9. Epub 2005 Feb 20. PMID:15723079
Page seeded by OCA on Thu Feb 21 16:37:12 2008
Categories: Bos taurus | Homo sapiens | Protein complex | Ubiquitin--protein ligase | Dijk, W J.Van. | Jentsch, S. | Knipscheer, P. | Korner, R. | Melchior, F. | Oberhofer, E. | Olsen, J Velgaard. | Pichler, A. | SPINE, Structural Proteomics in Europe. | Sixma, T K. | E2 ubiquitin conjugating enzyme | E2-25k | Ligase | Spine | Structural genomics | Structural proteomics in europe | Sumo | Sumo-target structure
