2bgy
From Proteopedia
(New page: 200px<br /><applet load="2bgy" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bgy" /> '''FIT OF THE X-RAY STRUCUTRE OF THE BATERIAL F...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2bgy.gif|left|200px]]<br /><applet load="2bgy" size=" | + | [[Image:2bgy.gif|left|200px]]<br /><applet load="2bgy" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2bgy" /> | caption="2bgy" /> | ||
'''FIT OF THE X-RAY STRUCUTRE OF THE BATERIAL FLAGELLAR HOOK FRAGMENT FLGE31 INTO AN EM MAP FROM THE HOOK OF CAULOBACTER CRESCENTUS.'''<br /> | '''FIT OF THE X-RAY STRUCUTRE OF THE BATERIAL FLAGELLAR HOOK FRAGMENT FLGE31 INTO AN EM MAP FROM THE HOOK OF CAULOBACTER CRESCENTUS.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The axial proteins of the bacterial flagellum function as a drive shaft, universal joint, and propeller driven by the flagellar rotary motor; they | + | The axial proteins of the bacterial flagellum function as a drive shaft, universal joint, and propeller driven by the flagellar rotary motor; they also form the putative protein export channel. The N- and C-terminal sequences of the eight axial proteins were predicted to form interlocking alpha-domains generating an axial tube. We report on an approximately 1-nm resolution map of the hook from Salmonella typhimurium, which reveals such a tube made from interdigitated, 1-nm rod-like densities similar to those seen in maps of the filament. Atomic models for the two outer domains of the hook subunit were docked into the corresponding outermost features of the map. The N and C termini of the hook subunit fragment are positioned next to each other and face toward the axis of the hook. The placement of these termini would permit the residues missing in the fragment to form the rod-like features that form the core domain of the hook. We also fit the hook atomic model to an approximately 2-nm resolution map of the hook from Caulobacter crescentus. The hook protein sequence from C. crescentus is largely homologous to that of S. typhimurium except for a large insertion (20 kDa). According to difference maps and our fitting, this insertion is found on the outer surface of the hook, consistent with our modeling of the hook. |
==About this Structure== | ==About this Structure== | ||
| - | 2BGY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http:// | + | 2BGY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BGY OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Salmonella typhimurium]] | [[Category: Salmonella typhimurium]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chen, J | + | [[Category: Chen, J Z.]] |
| - | [[Category: Derosier, D | + | [[Category: Derosier, D J.]] |
[[Category: Imada, K.]] | [[Category: Imada, K.]] | ||
[[Category: Matsunami, H.]] | [[Category: Matsunami, H.]] | ||
[[Category: Namba, K.]] | [[Category: Namba, K.]] | ||
| - | [[Category: Samatey, F | + | [[Category: Samatey, F A.]] |
| - | [[Category: Shaikh, T | + | [[Category: Shaikh, T R.]] |
| - | [[Category: Thomas, D | + | [[Category: Thomas, D R.]] |
[[Category: bacterial motility]] | [[Category: bacterial motility]] | ||
[[Category: electron microscopy]] | [[Category: electron microscopy]] | ||
| Line 27: | Line 27: | ||
[[Category: model building]] | [[Category: model building]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:37:40 2008'' |
Revision as of 14:37, 21 February 2008
|
FIT OF THE X-RAY STRUCUTRE OF THE BATERIAL FLAGELLAR HOOK FRAGMENT FLGE31 INTO AN EM MAP FROM THE HOOK OF CAULOBACTER CRESCENTUS.
Overview
The axial proteins of the bacterial flagellum function as a drive shaft, universal joint, and propeller driven by the flagellar rotary motor; they also form the putative protein export channel. The N- and C-terminal sequences of the eight axial proteins were predicted to form interlocking alpha-domains generating an axial tube. We report on an approximately 1-nm resolution map of the hook from Salmonella typhimurium, which reveals such a tube made from interdigitated, 1-nm rod-like densities similar to those seen in maps of the filament. Atomic models for the two outer domains of the hook subunit were docked into the corresponding outermost features of the map. The N and C termini of the hook subunit fragment are positioned next to each other and face toward the axis of the hook. The placement of these termini would permit the residues missing in the fragment to form the rod-like features that form the core domain of the hook. We also fit the hook atomic model to an approximately 2-nm resolution map of the hook from Caulobacter crescentus. The hook protein sequence from C. crescentus is largely homologous to that of S. typhimurium except for a large insertion (20 kDa). According to difference maps and our fitting, this insertion is found on the outer surface of the hook, consistent with our modeling of the hook.
About this Structure
2BGY is a Single protein structure of sequence from Salmonella typhimurium. Full crystallographic information is available from OCA.
Reference
A partial atomic structure for the flagellar hook of Salmonella typhimurium., Shaikh TR, Thomas DR, Chen JZ, Samatey FA, Matsunami H, Imada K, Namba K, Derosier DJ, Proc Natl Acad Sci U S A. 2005 Jan 25;102(4):1023-8. Epub 2005 Jan 18. PMID:15657146
Page seeded by OCA on Thu Feb 21 16:37:40 2008
