2bht
From Proteopedia
(New page: 200px<br /><applet load="2bht" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bht, resolution 2.10Å" /> '''CRYSTAL STRUCTURE OF...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2bht.gif|left|200px]]<br /><applet load="2bht" size=" | + | [[Image:2bht.gif|left|200px]]<br /><applet load="2bht" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2bht, resolution 2.10Å" /> | caption="2bht, resolution 2.10Å" /> | ||
'''CRYSTAL STRUCTURE OF O-ACETYLSERINE SULFHYDRYLASE B'''<br /> | '''CRYSTAL STRUCTURE OF O-ACETYLSERINE SULFHYDRYLASE B'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The enzyme O-acetylserine sulfhydrylase participates in the biosynthesis | + | The enzyme O-acetylserine sulfhydrylase participates in the biosynthesis of l-cysteine in bacteria and plants. The structure of isoenzyme B (CysM) from Escherichia coli was established in a hexagonal crystal form at 2.7 A resolution (wild-type) and in a merohedrally twinned tetragonal crystal form at 2.1 A resolution (surface mutant). Structural superpositions revealed the variations with respect to isoenzyme A (CysK) and explained the different substrate specificities. A geometric model of the reaction catalyzed by CysM is proposed. Both isoenzymes are used for the production of l-amino acid derivatives as building blocks for the synthesis of peptides and peptidomimetic drugs. Since the structure of CysM revealed a remarkable main chain variation at the active center, it constitutes a further starting point for engineering mutants with novel substrate specificities. |
==About this Structure== | ==About this Structure== | ||
| - | 2BHT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Cysteine_synthase Cysteine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.47 2.5.1.47] Full crystallographic information is available from [http:// | + | 2BHT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Cysteine_synthase Cysteine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.47 2.5.1.47] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BHT OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Claus, M | + | [[Category: Claus, M T.]] |
| - | [[Category: Maier, T | + | [[Category: Maier, T H.P.]] |
| - | [[Category: Schulz, G | + | [[Category: Schulz, G E.]] |
| - | [[Category: Zocher, G | + | [[Category: Zocher, G E.]] |
[[Category: cysteine biosynthesis]] | [[Category: cysteine biosynthesis]] | ||
[[Category: plp-dependent enzyme]] | [[Category: plp-dependent enzyme]] | ||
| Line 24: | Line 24: | ||
[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:37:57 2008'' |
Revision as of 14:38, 21 February 2008
|
CRYSTAL STRUCTURE OF O-ACETYLSERINE SULFHYDRYLASE B
Overview
The enzyme O-acetylserine sulfhydrylase participates in the biosynthesis of l-cysteine in bacteria and plants. The structure of isoenzyme B (CysM) from Escherichia coli was established in a hexagonal crystal form at 2.7 A resolution (wild-type) and in a merohedrally twinned tetragonal crystal form at 2.1 A resolution (surface mutant). Structural superpositions revealed the variations with respect to isoenzyme A (CysK) and explained the different substrate specificities. A geometric model of the reaction catalyzed by CysM is proposed. Both isoenzymes are used for the production of l-amino acid derivatives as building blocks for the synthesis of peptides and peptidomimetic drugs. Since the structure of CysM revealed a remarkable main chain variation at the active center, it constitutes a further starting point for engineering mutants with novel substrate specificities.
About this Structure
2BHT is a Single protein structure of sequence from Escherichia coli. Active as Cysteine synthase, with EC number 2.5.1.47 Full crystallographic information is available from OCA.
Reference
Structure of the O-acetylserine sulfhydrylase isoenzyme CysM from Escherichia coli., Claus MT, Zocher GE, Maier TH, Schulz GE, Biochemistry. 2005 Jun 21;44(24):8620-6. PMID:15952768
Page seeded by OCA on Thu Feb 21 16:37:57 2008
