3btn
From Proteopedia
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===Crystal structure of antizyme inhibitor, an ornithine decarboxylase homologous protein=== | ===Crystal structure of antizyme inhibitor, an ornithine decarboxylase homologous protein=== | ||
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==See Also== | ==See Also== | ||
*[[Antizyme Inhibitor|Antizyme Inhibitor]] | *[[Antizyme Inhibitor|Antizyme Inhibitor]] | ||
| + | *[[Ornithine decarboxylase|Ornithine decarboxylase]] | ||
==Reference== | ==Reference== | ||
Revision as of 10:44, 11 March 2013
Contents |
Crystal structure of antizyme inhibitor, an ornithine decarboxylase homologous protein
Antizyme inhibitor (AzI) regulates cellular polyamine homeostasis by binding to the polyamine-induced protein, Antizyme (Az), with greater affinity than ornithine decarboxylase (ODC). AzI is highly homologous to ODC but is not enzymatically active. In order to understand these specific characteristics of AzI and its differences from ODC, we determined the 3D structure of mouse AzI to 2.05 A resolution. Both AzI and ODC crystallize as a dimer. However, fewer interactions at the dimer interface, a smaller buried surface area, and lack of symmetry of the interactions between residues from the two monomers in the AzI structure suggest that this dimeric structure is nonphysiological. In addition, the absence of residues and interactions required for pyridoxal 5'-phosphate (PLP) binding suggests that AzI does not bind PLP. Biochemical studies confirmed the lack of PLP binding and revealed that AzI exists as a monomer in solution while ODC is dimeric. Our findings that AzI exists as a monomer and is unable to bind PLP provide two independent explanations for its lack of enzymatic activity and suggest the basis for its enhanced affinity toward Az.
Crystallographic and biochemical studies revealing the structural basis for antizyme inhibitor function., Albeck S, Dym O, Unger T, Snapir Z, Bercovich Z, Kahana C, Protein Sci. 2008 May;17(5):793-802. Epub 2008 Mar 27. PMID:18369191
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3btn is a 2 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA.
See Also
Reference
- Albeck S, Dym O, Unger T, Snapir Z, Bercovich Z, Kahana C. Crystallographic and biochemical studies revealing the structural basis for antizyme inhibitor function. Protein Sci. 2008 May;17(5):793-802. Epub 2008 Mar 27. PMID:18369191 doi:10.1110/ps.073427208
