2p1q
From Proteopedia
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{{STRUCTURE_2p1q| PDB=2p1q | SCENE= }} | {{STRUCTURE_2p1q| PDB=2p1q | SCENE= }} | ||
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===Mechanism of Auxin Perception by the TIR1 ubiquitin ligase=== | ===Mechanism of Auxin Perception by the TIR1 ubiquitin ligase=== | ||
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{{ABSTRACT_PUBMED_17410169}} | {{ABSTRACT_PUBMED_17410169}} | ||
Revision as of 10:57, 11 March 2013
Contents |
Mechanism of Auxin Perception by the TIR1 ubiquitin ligase
Auxin is a pivotal plant hormone that controls many aspects of plant growth and development. Perceived by a small family of F-box proteins including transport inhibitor response 1 (TIR1), auxin regulates gene expression by promoting SCF ubiquitin-ligase-catalysed degradation of the Aux/IAA transcription repressors, but how the TIR1 F-box protein senses and becomes activated by auxin remains unclear. Here we present the crystal structures of the Arabidopsis TIR1-ASK1 complex, free and in complexes with three different auxin compounds and an Aux/IAA substrate peptide. These structures show that the leucine-rich repeat domain of TIR1 contains an unexpected inositol hexakisphosphate co-factor and recognizes auxin and the Aux/IAA polypeptide substrate through a single surface pocket. Anchored to the base of the TIR1 pocket, auxin binds to a partially promiscuous site, which can also accommodate various auxin analogues. Docked on top of auxin, the Aux/IAA substrate peptide occupies the rest of the TIR1 pocket and completely encloses the hormone-binding site. By filling in a hydrophobic cavity at the protein interface, auxin enhances the TIR1-substrate interactions by acting as a 'molecular glue'. Our results establish the first structural model of a plant hormone receptor.
Mechanism of auxin perception by the TIR1 ubiquitin ligase., Tan X, Calderon-Villalobos LI, Sharon M, Zheng C, Robinson CV, Estelle M, Zheng N, Nature. 2007 Apr 5;446(7136):640-5. PMID:17410169
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2p1q is a 3 chain structure with sequence from Arabidopsis thaliana. The February 2009 RCSB PDB Molecule of the Month feature on Auxin and TIR1 Ubiquitin Ligase by David Goodsell is 10.2210/rcsb_pdb/mom_2009_2. Full crystallographic information is available from OCA.
See Also
Reference
- Tan X, Calderon-Villalobos LI, Sharon M, Zheng C, Robinson CV, Estelle M, Zheng N. Mechanism of auxin perception by the TIR1 ubiquitin ligase. Nature. 2007 Apr 5;446(7136):640-5. PMID:17410169 doi:10.1038/nature05731
