2bld
From Proteopedia
(New page: 200px<br /><applet load="2bld" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bld" /> '''THE QUASI-ATOMIC MODEL OF HUMAN ADENOVIRUS T...) |
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| - | [[Image:2bld.gif|left|200px]]<br /><applet load="2bld" size=" | + | [[Image:2bld.gif|left|200px]]<br /><applet load="2bld" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2bld" /> | caption="2bld" /> | ||
'''THE QUASI-ATOMIC MODEL OF HUMAN ADENOVIRUS TYPE 5 CAPSID (PART 1)'''<br /> | '''THE QUASI-ATOMIC MODEL OF HUMAN ADENOVIRUS TYPE 5 CAPSID (PART 1)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Adenoviruses infect a wide range of vertebrates including humans. Their | + | Adenoviruses infect a wide range of vertebrates including humans. Their icosahedral capsids are composed of three major proteins: the trimeric hexon forms the facets and the penton, a noncovalent complex of the pentameric penton base and trimeric fibre proteins, is located at the 12 capsid vertices. Several proteins (IIIa, VI, VIII and IX) stabilise the capsid. We have obtained a 10 A resolution map of the human adenovirus 5 by image analysis from cryo-electron micrographs (cryoEMs). This map, in combination with the X-ray structures of the penton base and hexon, was used to build a quasi-atomic model of the arrangement of the two major capsid components and to analyse the hexon-hexon and hexon-penton interactions. The secondary proteins, notably VIII, were located by comparing cryoEM maps of native and pIX deletion mutant virions. Minor proteins IX and IIIa are located on the outside of the capsid, whereas protein VIII is organised with a T=2 lattice on the inner face of the capsid. The capsid organisation is compared with the known X-ray structure of bacteriophage PRD1. |
==About this Structure== | ==About this Structure== | ||
| - | 2BLD is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Human_adenovirus_type_4 Human adenovirus type 4]. Full crystallographic information is available from [http:// | + | 2BLD is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Human_adenovirus_type_4 Human adenovirus type 4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BLD OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Human adenovirus type 4]] | [[Category: Human adenovirus type 4]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Conway, J | + | [[Category: Conway, J F.]] |
[[Category: Cusack, S.]] | [[Category: Cusack, S.]] | ||
| - | [[Category: Fabry, C | + | [[Category: Fabry, C M.S.]] |
[[Category: Rosa-Calatrava, M.]] | [[Category: Rosa-Calatrava, M.]] | ||
| - | [[Category: Ruigrok, R | + | [[Category: Ruigrok, R W.H.]] |
[[Category: Schoehn, G.]] | [[Category: Schoehn, G.]] | ||
[[Category: Zubieta, C.]] | [[Category: Zubieta, C.]] | ||
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[[Category: structure]] | [[Category: structure]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:39:01 2008'' |
Revision as of 14:39, 21 February 2008
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THE QUASI-ATOMIC MODEL OF HUMAN ADENOVIRUS TYPE 5 CAPSID (PART 1)
Overview
Adenoviruses infect a wide range of vertebrates including humans. Their icosahedral capsids are composed of three major proteins: the trimeric hexon forms the facets and the penton, a noncovalent complex of the pentameric penton base and trimeric fibre proteins, is located at the 12 capsid vertices. Several proteins (IIIa, VI, VIII and IX) stabilise the capsid. We have obtained a 10 A resolution map of the human adenovirus 5 by image analysis from cryo-electron micrographs (cryoEMs). This map, in combination with the X-ray structures of the penton base and hexon, was used to build a quasi-atomic model of the arrangement of the two major capsid components and to analyse the hexon-hexon and hexon-penton interactions. The secondary proteins, notably VIII, were located by comparing cryoEM maps of native and pIX deletion mutant virions. Minor proteins IX and IIIa are located on the outside of the capsid, whereas protein VIII is organised with a T=2 lattice on the inner face of the capsid. The capsid organisation is compared with the known X-ray structure of bacteriophage PRD1.
About this Structure
2BLD is a Protein complex structure of sequences from Human adenovirus type 4. Full crystallographic information is available from OCA.
Reference
A quasi-atomic model of human adenovirus type 5 capsid., Fabry CM, Rosa-Calatrava M, Conway JF, Zubieta C, Cusack S, Ruigrok RW, Schoehn G, EMBO J. 2005 May 4;24(9):1645-54. Epub 2005 Apr 21. PMID:15861131
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