2bm6
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Fluoroquinolones are gaining increasing importance in the treatment of | + | Fluoroquinolones are gaining increasing importance in the treatment of tuberculosis. The expression of MfpA, a member of the pentapeptide repeat family of proteins from Mycobacterium tuberculosis, causes resistance to ciprofloxacin and sparfloxacin. This protein binds to DNA gyrase and inhibits its activity. Its three-dimensional structure reveals a fold, which we have named the right-handed quadrilateral beta helix, that exhibits size, shape, and electrostatic similarity to B-form DNA. This represents a form of DNA mimicry and explains both its inhibitory effect on DNA gyrase and fluoroquinolone resistance resulting from the protein's expression in vivo. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Mycobacterium tuberculosis]] | [[Category: Mycobacterium tuberculosis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Blanchard, J | + | [[Category: Blanchard, J S.]] |
| - | [[Category: Hegde, S | + | [[Category: Hegde, S S.]] |
[[Category: Maxwell, A.]] | [[Category: Maxwell, A.]] | ||
| - | [[Category: Mitchenall, L | + | [[Category: Mitchenall, L A.]] |
| - | [[Category: Roderick, S | + | [[Category: Roderick, S L.]] |
| - | [[Category: Takiff, H | + | [[Category: Takiff, H E.]] |
| - | [[Category: Vetting, M | + | [[Category: Vetting, M W.]] |
[[Category: CS]] | [[Category: CS]] | ||
[[Category: dna gyrase]] | [[Category: dna gyrase]] | ||
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[[Category: right-handed quadrilateral beta-helix]] | [[Category: right-handed quadrilateral beta-helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:39:16 2008'' |
Revision as of 14:39, 21 February 2008
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THE STRUCTURE OF MFPA (RV3361C, C2221 CRYSTAL FORM). THE PENTAPEPTIDE REPEAT PROTEIN FROM MYCOBACTERIUM TUBERCULOSIS FOLDS AS A RIGHT-HANDED QUADRILATERAL BETA-HELIX.
Overview
Fluoroquinolones are gaining increasing importance in the treatment of tuberculosis. The expression of MfpA, a member of the pentapeptide repeat family of proteins from Mycobacterium tuberculosis, causes resistance to ciprofloxacin and sparfloxacin. This protein binds to DNA gyrase and inhibits its activity. Its three-dimensional structure reveals a fold, which we have named the right-handed quadrilateral beta helix, that exhibits size, shape, and electrostatic similarity to B-form DNA. This represents a form of DNA mimicry and explains both its inhibitory effect on DNA gyrase and fluoroquinolone resistance resulting from the protein's expression in vivo.
About this Structure
2BM6 is a Single protein structure of sequence from Mycobacterium tuberculosis with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
A fluoroquinolone resistance protein from Mycobacterium tuberculosis that mimics DNA., Hegde SS, Vetting MW, Roderick SL, Mitchenall LA, Maxwell A, Takiff HE, Blanchard JS, Science. 2005 Jun 3;308(5727):1480-3. PMID:15933203
Page seeded by OCA on Thu Feb 21 16:39:16 2008
