2bmj
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Centaurins are a family of proteins that contain GTPase-activating protein | + | Centaurins are a family of proteins that contain GTPase-activating protein domains, with the gamma family members containing in addition a GTPase-like domain. Centaurins reside mainly in the nucleus and are known to activate phosphoinositide 3-kinase, a key regulator of cell proliferation, motility and vesicular trafficking. In the present study, using X-ray structural analysis, enzymatic assays and nucleotide-binding studies, we show that, for CENTG1 (centaurin gamma-1) the GTPase-like domain has broader trinucleotide specificity. Alterations within the G4 motif of CENTG1 from the highly conserved NKXD found in typical GTPases to TQDR result in the loss of specificity, a lower affinity for the nucleotides and higher turnover rates. These results indicate that the centaurins could be more accurately classified as NTPases and point to alternative mechanisms of cell signalling control. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Arrowsmith, C.]] | [[Category: Arrowsmith, C.]] | ||
| - | [[Category: Doyle, D | + | [[Category: Doyle, D A.]] |
[[Category: Edwards, A.]] | [[Category: Edwards, A.]] | ||
| - | [[Category: Elkins, J | + | [[Category: Elkins, J M.]] |
| - | [[Category: Sgc, Structural | + | [[Category: Sgc, Structural Genomics Consortium.]] |
[[Category: Soundararajan, M.]] | [[Category: Soundararajan, M.]] | ||
[[Category: Sundstrom, M.]] | [[Category: Sundstrom, M.]] | ||
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[[Category: structural genomics consortium]] | [[Category: structural genomics consortium]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:39:22 2008'' |
Revision as of 14:39, 21 February 2008
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GTPASE LIKE DOMAIN OF CENTAURIN GAMMA 1 (HUMAN)
Overview
Centaurins are a family of proteins that contain GTPase-activating protein domains, with the gamma family members containing in addition a GTPase-like domain. Centaurins reside mainly in the nucleus and are known to activate phosphoinositide 3-kinase, a key regulator of cell proliferation, motility and vesicular trafficking. In the present study, using X-ray structural analysis, enzymatic assays and nucleotide-binding studies, we show that, for CENTG1 (centaurin gamma-1) the GTPase-like domain has broader trinucleotide specificity. Alterations within the G4 motif of CENTG1 from the highly conserved NKXD found in typical GTPases to TQDR result in the loss of specificity, a lower affinity for the nucleotides and higher turnover rates. These results indicate that the centaurins could be more accurately classified as NTPases and point to alternative mechanisms of cell signalling control.
About this Structure
2BMJ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The centaurin gamma-1 GTPase-like domain functions as an NTPase., Soundararajan M, Yang X, Elkins JM, Sobott F, Doyle DA, Biochem J. 2007 Feb 1;401(3):679-88. PMID:17037982
Page seeded by OCA on Thu Feb 21 16:39:22 2008
