2bn6
From Proteopedia
(New page: 200px<br /><applet load="2bn6" size="350" color="white" frame="true" align="right" spinBox="true" caption="2bn6" /> '''P-ELEMENT SOMATIC INHIBITOR PROTEIN'''<br />...) |
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==Overview== | ==Overview== | ||
| - | P-element transposition in Drosophila is regulated by tissue-specific | + | P-element transposition in Drosophila is regulated by tissue-specific alternative splicing of the P-element transposase pre-mRNA. In somatic cells, the P-element somatic inhibitor (PSI) protein binds to exon 3 of the pre-mRNA and recruits U1 small nuclear ribonucleoprotein (snRNP) to the F1 pseudo-splice site. This abrogates binding of U1 snRNP to the genuine 5' splice site, thereby preventing excision of the third intron. Two homologous short sequences, referred to as the A and B boxes, near the C terminus of PSI bind to U1-70k protein within U1 snRNP. We have now mapped the AB box-binding site of U1-70k to a short proline-rich sequence at the C terminus. Our NMR study shows that the B box forms an anti-parallel helical hairpin in which four highly conserved aromatic residues form a cluster on one face of the first helix. This hydrophobic cluster interacts extensively with the proline-rich region of the U1-70k protein. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Butler, P | + | [[Category: Butler, P J.G.]] |
[[Category: Ignjatovic, T.]] | [[Category: Ignjatovic, T.]] | ||
[[Category: Nagai, K.]] | [[Category: Nagai, K.]] | ||
[[Category: Neuhaus, D.]] | [[Category: Neuhaus, D.]] | ||
| - | [[Category: Yang, J | + | [[Category: Yang, J C.]] |
[[Category: nmr structure]] | [[Category: nmr structure]] | ||
[[Category: nuclear protein]] | [[Category: nuclear protein]] | ||
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[[Category: splicing]] | [[Category: splicing]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:39:32 2008'' |
Revision as of 14:39, 21 February 2008
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P-ELEMENT SOMATIC INHIBITOR PROTEIN
Overview
P-element transposition in Drosophila is regulated by tissue-specific alternative splicing of the P-element transposase pre-mRNA. In somatic cells, the P-element somatic inhibitor (PSI) protein binds to exon 3 of the pre-mRNA and recruits U1 small nuclear ribonucleoprotein (snRNP) to the F1 pseudo-splice site. This abrogates binding of U1 snRNP to the genuine 5' splice site, thereby preventing excision of the third intron. Two homologous short sequences, referred to as the A and B boxes, near the C terminus of PSI bind to U1-70k protein within U1 snRNP. We have now mapped the AB box-binding site of U1-70k to a short proline-rich sequence at the C terminus. Our NMR study shows that the B box forms an anti-parallel helical hairpin in which four highly conserved aromatic residues form a cluster on one face of the first helix. This hydrophobic cluster interacts extensively with the proline-rich region of the U1-70k protein.
About this Structure
2BN6 is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Structural basis of the interaction between P-element somatic inhibitor and U1-70k essential for the alternative splicing of P-element transposase., Ignjatovic T, Yang JC, Butler J, Neuhaus D, Nagai K, J Mol Biol. 2005 Aug 5;351(1):52-65. PMID:15990112
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