2bog
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Endoglucanase Cel6A from Thermobifida fusca hydrolyzes the beta-1,4 | + | Endoglucanase Cel6A from Thermobifida fusca hydrolyzes the beta-1,4 linkages in cellulose at accessible points along the polymer. The structure of the catalytic domain of Cel6A from T. fusca in complex with a nonhydrolysable substrate analogue that acts as an inhibitor, methylcellobiosyl-4-thio-beta-cellobioside (Glc(2)-S-Glc(2)), has been determined to 1.5 A resolution. The glycosyl unit in subsite -1 was sterically hindered by Tyr73 and forced into a distorted (2)S(o) conformation. In the enzyme where Tyr73 was mutated to a serine residue, the hindrance was removed and the glycosyl unit in subsite -1 had a relaxed (4)C(1) chair conformation. The relaxed conformation was seen in two complex structures of the mutated enzyme, with cellotetrose (Glc(4)) at 1.64 A and Glc(2)-S-Glc(2) at 1.04 A resolution. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Driguez, H.]] | [[Category: Driguez, H.]] | ||
[[Category: Dultz, E.]] | [[Category: Dultz, E.]] | ||
| - | [[Category: Irwin, D | + | [[Category: Irwin, D C.]] |
| - | [[Category: Jones, T | + | [[Category: Jones, T A.]] |
| - | [[Category: Larsson, A | + | [[Category: Larsson, A M.]] |
[[Category: Roos, A.]] | [[Category: Roos, A.]] | ||
| - | [[Category: Wilson, D | + | [[Category: Wilson, D B.]] |
[[Category: endoglucanase]] | [[Category: endoglucanase]] | ||
[[Category: glycoside hydrolase family 6]] | [[Category: glycoside hydrolase family 6]] | ||
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[[Category: tim a/b fold]] | [[Category: tim a/b fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:39:57 2008'' |
Revision as of 14:39, 21 February 2008
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CATALYTIC DOMAIN OF ENDO-1,4-GLUCANASE CEL6A MUTANT Y73S FROM THERMOBIFIDA FUSCA IN COMPLEX WITH METHYL CELLOBIOSYL-4-THIO-BETA-CELLOBIOSIDE
Overview
Endoglucanase Cel6A from Thermobifida fusca hydrolyzes the beta-1,4 linkages in cellulose at accessible points along the polymer. The structure of the catalytic domain of Cel6A from T. fusca in complex with a nonhydrolysable substrate analogue that acts as an inhibitor, methylcellobiosyl-4-thio-beta-cellobioside (Glc(2)-S-Glc(2)), has been determined to 1.5 A resolution. The glycosyl unit in subsite -1 was sterically hindered by Tyr73 and forced into a distorted (2)S(o) conformation. In the enzyme where Tyr73 was mutated to a serine residue, the hindrance was removed and the glycosyl unit in subsite -1 had a relaxed (4)C(1) chair conformation. The relaxed conformation was seen in two complex structures of the mutated enzyme, with cellotetrose (Glc(4)) at 1.64 A and Glc(2)-S-Glc(2) at 1.04 A resolution.
About this Structure
2BOG is a Single protein structure of sequence from Thermobifida fusca. Active as Cellulase, with EC number 3.2.1.4 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystal structure of Thermobifida fusca endoglucanase Cel6A in complex with substrate and inhibitor: the role of tyrosine Y73 in substrate ring distortion., Larsson AM, Bergfors T, Dultz E, Irwin DC, Roos A, Driguez H, Wilson DB, Jones TA, Biochemistry. 2005 Oct 4;44(39):12915-22. PMID:16185060
Page seeded by OCA on Thu Feb 21 16:39:57 2008
