2brr
From Proteopedia
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==Overview== | ==Overview== | ||
| - | In various western countries, subtype P1.4 of Neisseria meningitidis | + | In various western countries, subtype P1.4 of Neisseria meningitidis serogroup B causes the greatest incidence of meningococcal disease. To investigate the molecular recognition of this subtype, we crystallised a peptide (P1HVVVNNKVATH(P11)), corresponding to the subtype P1.4 epitope sequence of outer membrane protein PorA, in complex with a Fab fragment of the bactericidal antibody MN20B9.34 directed against this epitope. Structure determination at 1.95 A resolution revealed a unique complex of one P1.4 antigen peptide bound to two identical Fab fragments. One Fab recognises the putative epitope residues in a 2:2 type I beta-turn at residues P5NNKV(P8), whereas the other Fab binds the C-terminal residues of the peptide that we consider a crystallisation artefact. Interestingly, recognition of the P1.4 epitope peptide is mediated almost exclusively through the complementarity-determining regions of the heavy chain. We exploited the observed turn conformation for designing conformationally restricted cyclic peptides for use as a peptide vaccine. The conformational stability of the two peptide designs was assessed by molecular dynamics simulations. Unlike the linear peptide, both cyclic peptides, conjugated to tetanus toxoid as a carrier protein, elicited antibody responses in mice that recognised meningococci of subtype P1.7-2,4. Serum bactericidal assays showed that some, but not all, of the sera induced with the cyclic peptide conjugates could activate the complement system with titres that were very high compared to the titres induced by complete PorA protein in its native conformation administered in outer membrane vesicles. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Alphen, L | + | [[Category: Alphen, L Van.]] |
[[Category: Gros, P.]] | [[Category: Gros, P.]] | ||
[[Category: Hoogerhout, P.]] | [[Category: Hoogerhout, P.]] | ||
[[Category: Kuipers, B.]] | [[Category: Kuipers, B.]] | ||
| - | [[Category: Oomen, C | + | [[Category: Oomen, C J.]] |
[[Category: Vidarsson, G.]] | [[Category: Vidarsson, G.]] | ||
[[Category: ACY]] | [[Category: ACY]] | ||
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[[Category: antigen]] | [[Category: antigen]] | ||
[[Category: fab fragment]] | [[Category: fab fragment]] | ||
| - | [[Category: p1 | + | [[Category: p1 4 antibody]] |
[[Category: porin]] | [[Category: porin]] | ||
[[Category: transmembrane]] | [[Category: transmembrane]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:41:00 2008'' |
Revision as of 14:41, 21 February 2008
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COMPLEX OF THE NEISSERIAL PORA P1.4 EPITOPE PEPTIDE AND TWO FAB-FRAGMENTS (ANTIBODY MN20B9.34)
Overview
In various western countries, subtype P1.4 of Neisseria meningitidis serogroup B causes the greatest incidence of meningococcal disease. To investigate the molecular recognition of this subtype, we crystallised a peptide (P1HVVVNNKVATH(P11)), corresponding to the subtype P1.4 epitope sequence of outer membrane protein PorA, in complex with a Fab fragment of the bactericidal antibody MN20B9.34 directed against this epitope. Structure determination at 1.95 A resolution revealed a unique complex of one P1.4 antigen peptide bound to two identical Fab fragments. One Fab recognises the putative epitope residues in a 2:2 type I beta-turn at residues P5NNKV(P8), whereas the other Fab binds the C-terminal residues of the peptide that we consider a crystallisation artefact. Interestingly, recognition of the P1.4 epitope peptide is mediated almost exclusively through the complementarity-determining regions of the heavy chain. We exploited the observed turn conformation for designing conformationally restricted cyclic peptides for use as a peptide vaccine. The conformational stability of the two peptide designs was assessed by molecular dynamics simulations. Unlike the linear peptide, both cyclic peptides, conjugated to tetanus toxoid as a carrier protein, elicited antibody responses in mice that recognised meningococci of subtype P1.7-2,4. Serum bactericidal assays showed that some, but not all, of the sera induced with the cyclic peptide conjugates could activate the complement system with titres that were very high compared to the titres induced by complete PorA protein in its native conformation administered in outer membrane vesicles.
About this Structure
2BRR is a Protein complex structure of sequences from Mus musculus with , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystal structure of an Anti-meningococcal subtype P1.4 PorA antibody provides basis for peptide-vaccine design., Oomen CJ, Hoogerhout P, Kuipers B, Vidarsson G, van Alphen L, Gros P, J Mol Biol. 2005 Sep 2;351(5):1070-80. PMID:16038932
Page seeded by OCA on Thu Feb 21 16:41:00 2008
Categories: Mus musculus | Protein complex | Alphen, L Van. | Gros, P. | Hoogerhout, P. | Kuipers, B. | Oomen, C J. | Vidarsson, G. | ACY | NH2 | SO4 | Anti-pora antibody | Antibody/antigen complex | Antigen | Fab fragment | P1 4 antibody | Porin | Transmembrane
