This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2bt3
From Proteopedia
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | Molecular wires comprising a Ru(II)- or Re(I)-complex head group, an | + | Molecular wires comprising a Ru(II)- or Re(I)-complex head group, an aromatic tail group, and an alkane linker reversibly inhibit the activity of the copper amine oxidase from Arthrobacter globiformis (AGAO), with K(i) values between 6 muM and 37 nM. In the crystal structure of a Ru(II)-wire:AGAO conjugate, the wire occupies the AGAO active-site substrate access channel, the trihydroxyphenylalanine quinone cofactor is ordered in the "off-Cu" position with its reactive carbonyl oriented toward the inhibitor, and the "gate" residue, Tyr-296, is in the "open" position. Head groups, tail-group substituents, and linker lengths all influence wire-binding interactions with the enzyme. |
==About this Structure== | ==About this Structure== | ||
| Line 14: | Line 14: | ||
[[Category: Arthrobacter globiformis]] | [[Category: Arthrobacter globiformis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Contakes, S | + | [[Category: Contakes, S M.]] |
| - | [[Category: Dooley, D | + | [[Category: Dooley, D M.]] |
| - | [[Category: Duff, A | + | [[Category: Duff, A P.]] |
| - | [[Category: Dunn, A | + | [[Category: Dunn, A R.]] |
| - | [[Category: Freeman, H | + | [[Category: Freeman, H C.]] |
| - | [[Category: Gray, H | + | [[Category: Gray, H B.]] |
| - | [[Category: Guss, J | + | [[Category: Guss, J M.]] |
| - | [[Category: Halpern-Manners, N | + | [[Category: Halpern-Manners, N W.]] |
| - | [[Category: Juda, G | + | [[Category: Juda, G A.]] |
| - | [[Category: Langley, D | + | [[Category: Langley, D B.]] |
[[Category: CU]] | [[Category: CU]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
| Line 40: | Line 40: | ||
[[Category: tpq]] | [[Category: tpq]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:41:20 2008'' |
Revision as of 14:41, 21 February 2008
|
AGAO IN COMPLEX WITH RUTHENIUM-C4-WIRE AT 1.73 ANGSTROMS
Overview
Molecular wires comprising a Ru(II)- or Re(I)-complex head group, an aromatic tail group, and an alkane linker reversibly inhibit the activity of the copper amine oxidase from Arthrobacter globiformis (AGAO), with K(i) values between 6 muM and 37 nM. In the crystal structure of a Ru(II)-wire:AGAO conjugate, the wire occupies the AGAO active-site substrate access channel, the trihydroxyphenylalanine quinone cofactor is ordered in the "off-Cu" position with its reactive carbonyl oriented toward the inhibitor, and the "gate" residue, Tyr-296, is in the "open" position. Head groups, tail-group substituents, and linker lengths all influence wire-binding interactions with the enzyme.
About this Structure
2BT3 is a Single protein structure of sequence from Arthrobacter globiformis with , , , and as ligands. Active as Amine oxidase (copper-containing), with EC number 1.4.3.6 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Reversible inhibition of copper amine oxidase activity by channel-blocking ruthenium(II) and rhenium(I) molecular wires., Contakes SM, Juda GA, Langley DB, Halpern-Manners NW, Duff AP, Dunn AR, Gray HB, Dooley DM, Guss JM, Freeman HC, Proc Natl Acad Sci U S A. 2005 Sep 20;102(38):13451-6. Epub 2005 Sep 12. PMID:16157884
Page seeded by OCA on Thu Feb 21 16:41:20 2008
Categories: Amine oxidase (copper-containing) | Arthrobacter globiformis | Single protein | Contakes, S M. | Dooley, D M. | Duff, A P. | Dunn, A R. | Freeman, H C. | Gray, H B. | Guss, J M. | Halpern-Manners, N W. | Juda, G A. | Langley, D B. | CU | GOL | NA | R4A | SO4 | 3d-structure | Amine oxidase | Competitive inhibition | Copper | Copper containing | Oxidoreductase | Quinone | Ruthenium diimine wires | Tpq
