2buo
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Immature HIV particles bud from infected cells after assembly at the | + | Immature HIV particles bud from infected cells after assembly at the cytoplasmic side of cellular membranes. This assembly is driven by interactions between Gag polyproteins. Mature particles, each containing a characteristic conical core, are later generated by proteolytic maturation of Gag in the virion. The C-terminal domain of the HIV-1 capsid protein (C-CA) has been shown to contain oligomerization determinants essential for particle assembly. Here we report the 1.7-A-resolution crystal structure of C-CA in complex with a peptide capable of inhibiting immature- and mature-like particle assembly in vitro. The peptide inserts as an amphipathic alpha-helix into a conserved hydrophobic groove of C-CA, resulting in formation of a compact five-helix bundle with altered dimeric interactions. This structure thus reveals the details of an allosteric site in the HIV capsid protein that can be targeted for antiviral therapy. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Duquerroy, S.]] | [[Category: Duquerroy, S.]] | ||
| - | [[Category: Krausslich, H | + | [[Category: Krausslich, H G.]] |
| - | [[Category: Rey, F | + | [[Category: Rey, F A.]] |
[[Category: Sticht, J.]] | [[Category: Sticht, J.]] | ||
[[Category: Ternois, F.]] | [[Category: Ternois, F.]] | ||
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[[Category: polyprotein]] | [[Category: polyprotein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:41:48 2008'' |
Revision as of 14:41, 21 February 2008
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HIV-1 CAPSID C-TERMINAL DOMAIN IN COMPLEX WITH AN INHIBITOR OF PARTICLE ASSEMBLY
Overview
Immature HIV particles bud from infected cells after assembly at the cytoplasmic side of cellular membranes. This assembly is driven by interactions between Gag polyproteins. Mature particles, each containing a characteristic conical core, are later generated by proteolytic maturation of Gag in the virion. The C-terminal domain of the HIV-1 capsid protein (C-CA) has been shown to contain oligomerization determinants essential for particle assembly. Here we report the 1.7-A-resolution crystal structure of C-CA in complex with a peptide capable of inhibiting immature- and mature-like particle assembly in vitro. The peptide inserts as an amphipathic alpha-helix into a conserved hydrophobic groove of C-CA, resulting in formation of a compact five-helix bundle with altered dimeric interactions. This structure thus reveals the details of an allosteric site in the HIV capsid protein that can be targeted for antiviral therapy.
About this Structure
2BUO is a Protein complex structure of sequences from Human immunodeficiency virus 1 with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The HIV-1 capsid protein C-terminal domain in complex with a virus assembly inhibitor., Ternois F, Sticht J, Duquerroy S, Krausslich HG, Rey FA, Nat Struct Mol Biol. 2005 Aug;12(8):678-82. Epub 2005 Jul 24. PMID:16041386
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