2bze
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The yeast Paf1 complex consists of Paf1, Rtf1, Cdc73, Ctr9, and Leo1 and | + | The yeast Paf1 complex consists of Paf1, Rtf1, Cdc73, Ctr9, and Leo1 and regulates histone H2B ubiquitination, histone H3 methylation, RNA polymerase II carboxy-terminal domain (CTD) Ser2 phosphorylation, and RNA 3' end processing. We provide structural insight into the Paf1 complex with the NMR structure of the conserved and functionally important Plus3 domain of human Rtf1. A predominantly beta-stranded subdomain displays structural similarity to Dicer/Argonaute PAZ domains and to Tudor domains. We further demonstrate that the highly basic Rtf1 Plus3 domain can interact in vitro with single-stranded DNA via residues on the rim of the beta sheet, reminiscent of siRNA binding by PAZ domains, but did not detect binding to double-stranded DNA or RNA. We discuss the potential role of Rtf1 Plus3 ssDNA binding during transcription elongation. |
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
| - | Structure and DNA binding of the human | + | Structure and DNA binding of the human Rtf1 Plus3 domain., de Jong RN, Truffault V, Diercks T, Ab E, Daniels MA, Kaptein R, Folkers GE, Structure. 2008 Jan;16(1):149-59. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18184592 18184592] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Ab, E.]] | [[Category: Ab, E.]] | ||
| - | [[Category: Daniels, M | + | [[Category: Daniels, M A.]] |
[[Category: Diercks, T.]] | [[Category: Diercks, T.]] | ||
| - | [[Category: Folkers, G | + | [[Category: Folkers, G E.]] |
| - | [[Category: Jong, R | + | [[Category: Jong, R N.De.]] |
[[Category: Kaptein, R.]] | [[Category: Kaptein, R.]] | ||
| - | [[Category: Spine, Structural | + | [[Category: Spine, Structural Proteomics in Europe.]] |
[[Category: Truffault, V.]] | [[Category: Truffault, V.]] | ||
[[Category: cdc73]] | [[Category: cdc73]] | ||
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[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:43:14 2008'' |
Revision as of 14:43, 21 February 2008
|
NMR STRUCTURE OF HUMAN RTF1 PLUS3 DOMAIN.
Overview
The yeast Paf1 complex consists of Paf1, Rtf1, Cdc73, Ctr9, and Leo1 and regulates histone H2B ubiquitination, histone H3 methylation, RNA polymerase II carboxy-terminal domain (CTD) Ser2 phosphorylation, and RNA 3' end processing. We provide structural insight into the Paf1 complex with the NMR structure of the conserved and functionally important Plus3 domain of human Rtf1. A predominantly beta-stranded subdomain displays structural similarity to Dicer/Argonaute PAZ domains and to Tudor domains. We further demonstrate that the highly basic Rtf1 Plus3 domain can interact in vitro with single-stranded DNA via residues on the rim of the beta sheet, reminiscent of siRNA binding by PAZ domains, but did not detect binding to double-stranded DNA or RNA. We discuss the potential role of Rtf1 Plus3 ssDNA binding during transcription elongation.
About this Structure
2BZE is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure and DNA binding of the human Rtf1 Plus3 domain., de Jong RN, Truffault V, Diercks T, Ab E, Daniels MA, Kaptein R, Folkers GE, Structure. 2008 Jan;16(1):149-59. PMID:18184592
Page seeded by OCA on Thu Feb 21 16:43:14 2008
Categories: Homo sapiens | Single protein | Ab, E. | Daniels, M A. | Diercks, T. | Folkers, G E. | Jong, R N.De. | Kaptein, R. | Spine, Structural Proteomics in Europe. | Truffault, V. | Cdc73 | Ctr9 | Elongation | H2b ubiquitination | Histone h3 methylation | Human rtf1 plus3 domain | Leo1 | Paf1 complex | Plus3 domain | Spine | Structural genomics | Structural proteomics in europe | Transcription | Transcription regulation
