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1obf

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[[Category: oxidoreductase]]
[[Category: oxidoreductase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:11:57 2007''
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:48:55 2007''

Revision as of 13:44, 30 October 2007

Image:1obf.gif

1obf, resolution 1.7Å

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THE CRYSTAL STRUCTURE OF GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE FROM ALCALIGENES XYLOSOXIDANS AT 1.7 RESOLUTION.

Overview

The enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from the, Gram-negative denitrifying bacterial species Alcaligenes xylosoxidans was, purified and crystallized as a contaminant protein during purification of, nitrous oxide reductase. This is the first structure of a GAPDH from a, denitrifying species. The crystal structure was solved at 1.7 A resolution, by molecular replacement using the structure of GAPDH from Bacillus, stearothermophilus as a starting model. The quality of the structure, enabled the amino-acid sequence of the A. xylosoxidans GAPDH to be, assigned. The structure is that of the apo-enzyme, lacking the NAD+, cofactor and with the active-site residue Cys154 oxidized. The global, structure of the enzyme has a homotetrameric quaternary structure similar, to that ... [(full description)]

About this Structure

1OBF is a [Single protein] structure of sequence from [Achromobacter xylosoxidans] with SO4, K and PG4 as [ligands]. Active as [Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)], with EC number [1.2.1.12]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

The structure of glyceraldehyde 3-phosphate dehydrogenase from Alcaligenes xylosoxidans at 1.7 A resolution., Antonyuk SV, Eady RR, Strange RW, Hasnain SS, Acta Crystallogr D Biol Crystallogr. 2003 May;59(Pt 5):835-42. Epub 2003, Apr 25. PMID:12777799

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