2c5b
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The fluorinase enzyme from Streptomyces cattleya displays an unusual | + | The fluorinase enzyme from Streptomyces cattleya displays an unusual ability in biocatalysis in that it forms a C-F bond. We now report that the enzyme will accept 2'-deoxyadenosine in place of adenosine substrates, and structural evidence reveals a reorganisation in hydrogen bonding to accommodate this substrate series. It emerges from this study that the enzyme does not require a planar ribose conformation of the substrate to catalyse C-F bond formation. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Streptomyces cattleya]] | [[Category: Streptomyces cattleya]] | ||
[[Category: Deng, H.]] | [[Category: Deng, H.]] | ||
| - | [[Category: Hagan, D | + | [[Category: Hagan, D O.]] |
| - | [[Category: Mcewan, A | + | [[Category: Mcewan, A R.]] |
| - | [[Category: Mcglinchey, R | + | [[Category: Mcglinchey, R P.]] |
| - | [[Category: Naismith, J | + | [[Category: Naismith, J H.]] |
| - | [[Category: Robinson, D | + | [[Category: Robinson, D A.]] |
[[Category: Spencer, J.]] | [[Category: Spencer, J.]] | ||
[[Category: 5F1]] | [[Category: 5F1]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:45:01 2008'' |
Revision as of 14:45, 21 February 2008
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X-RAY CRYSTAL STRUCTURE OF 5'-FLUORODEOXYADENOSINE SYNTHASE FROM STREPTOMYCES CATTLEYA COMPLEXED WITH 2'DEOXY-5'DEOXY-FLUOROADENOSINE.
Overview
The fluorinase enzyme from Streptomyces cattleya displays an unusual ability in biocatalysis in that it forms a C-F bond. We now report that the enzyme will accept 2'-deoxyadenosine in place of adenosine substrates, and structural evidence reveals a reorganisation in hydrogen bonding to accommodate this substrate series. It emerges from this study that the enzyme does not require a planar ribose conformation of the substrate to catalyse C-F bond formation.
About this Structure
2C5B is a Single protein structure of sequence from Streptomyces cattleya with and as ligands. Active as Adenosyl-fluoride synthase, with EC number 2.5.1.63 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates., Cobb SL, Deng H, McEwan AR, Naismith JH, O'Hagan D, Robinson DA, Org Biomol Chem. 2006 Apr 21;4(8):1458-60. Epub 2006 Mar 8. PMID:16604208
Page seeded by OCA on Thu Feb 21 16:45:01 2008
