2ce9
From Proteopedia
(New page: 200px<br /> <applet load="2ce9" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ce9, resolution 2.12Å" /> '''A WRPW PEPTIDE BOUN...) |
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| - | [[Image:2ce9.gif|left|200px]]<br /> | + | [[Image:2ce9.gif|left|200px]]<br /><applet load="2ce9" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2ce9" size=" | + | |
caption="2ce9, resolution 2.12Å" /> | caption="2ce9, resolution 2.12Å" /> | ||
'''A WRPW PEPTIDE BOUND TO THE GROUCHO-TLE WD40 DOMAIN.'''<br /> | '''A WRPW PEPTIDE BOUND TO THE GROUCHO-TLE WD40 DOMAIN.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Groucho (Gro)/TLE/Grg family of corepressors operates in many | + | The Groucho (Gro)/TLE/Grg family of corepressors operates in many signaling pathways (including Notch and Wnt). Gro/TLE proteins recognize a wide range of transcriptional repressors by binding to divergent short peptide sequences, including a C-terminal WRPW/Y motif (Hairy/Hes/Runx) and internal eh1 motifs (FxIxxIL; Engrailed/Goosecoid/Pax/Nkx). Here, we identify several missense mutations in Drosophila Gro, which demonstrate peptide binding to the central pore of the WD (WD40) beta propeller domain in vitro and in vivo. We define these interactions at the molecular level with crystal structures of the WD domain of human TLE1 bound to either WRPW or eh1 peptides. The two distinct peptide motifs adopt markedly different bound conformations but occupy overlapping sites across the central pore of the beta propeller. Our structural and functional analysis explains the rigid conservation of the WRPW motif, the sequence flexibility of eh1 motifs, and other aspects of repressor recognition by Gro in vivo. |
==About this Structure== | ==About this Structure== | ||
| - | 2CE9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 2CE9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CE9 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Pearl, L | + | [[Category: Pearl, L H.]] |
| - | [[Category: Pickles, L | + | [[Category: Pickles, L M.]] |
| - | [[Category: Roe, S | + | [[Category: Roe, S M.]] |
[[Category: nuclear protein]] | [[Category: nuclear protein]] | ||
[[Category: phosphorylation]] | [[Category: phosphorylation]] | ||
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[[Category: wnt signaling pathway]] | [[Category: wnt signaling pathway]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:47:44 2008'' |
Revision as of 14:47, 21 February 2008
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A WRPW PEPTIDE BOUND TO THE GROUCHO-TLE WD40 DOMAIN.
Overview
The Groucho (Gro)/TLE/Grg family of corepressors operates in many signaling pathways (including Notch and Wnt). Gro/TLE proteins recognize a wide range of transcriptional repressors by binding to divergent short peptide sequences, including a C-terminal WRPW/Y motif (Hairy/Hes/Runx) and internal eh1 motifs (FxIxxIL; Engrailed/Goosecoid/Pax/Nkx). Here, we identify several missense mutations in Drosophila Gro, which demonstrate peptide binding to the central pore of the WD (WD40) beta propeller domain in vitro and in vivo. We define these interactions at the molecular level with crystal structures of the WD domain of human TLE1 bound to either WRPW or eh1 peptides. The two distinct peptide motifs adopt markedly different bound conformations but occupy overlapping sites across the central pore of the beta propeller. Our structural and functional analysis explains the rigid conservation of the WRPW motif, the sequence flexibility of eh1 motifs, and other aspects of repressor recognition by Gro in vivo.
About this Structure
2CE9 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Molecular recognition of transcriptional repressor motifs by the WD domain of the Groucho/TLE corepressor., Jennings BH, Pickles LM, Wainwright SM, Roe SM, Pearl LH, Ish-Horowicz D, Mol Cell. 2006 Jun 9;22(5):645-55. PMID:16762837
Page seeded by OCA on Thu Feb 21 16:47:44 2008
