2chv
From Proteopedia
(New page: 200px<br /><applet load="2chv" size="450" color="white" frame="true" align="right" spinBox="true" caption="2chv, resolution 4.00Å" /> '''REPLICATION FACTOR C...) |
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| - | [[Image:2chv.gif|left|200px]]<br /><applet load="2chv" size=" | + | [[Image:2chv.gif|left|200px]]<br /><applet load="2chv" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2chv, resolution 4.00Å" /> | caption="2chv, resolution 4.00Å" /> | ||
'''REPLICATION FACTOR C ADPNP COMPLEX'''<br /> | '''REPLICATION FACTOR C ADPNP COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We have investigated the communication between subunits in replication | + | We have investigated the communication between subunits in replication factor C (RFC) from Archaeoglobus fulgidus. Mutation of the proposed arginine finger in the small subunits results in a complex that can still bind ATP but has impaired clamp-loading activity, a process that normally only requires binding of nucleotide. The small subunit alone forms a hexameric ring that is six-fold symmetric in the absence of ATP. However, this symmetry is broken when the nucleotide is bound to the complex. A conformational change associated with nucleotide binding may relate to the opening of PCNA rings by RFC during the loading reaction. The structures also reveal the importance of the N-terminal helix of each subunit at the ATP-binding site. Analysis of mutant protein complexes containing subunits lacking this N-terminal helix reveals key distinct regulatory roles during clamp loading that are different for the large and small subunits in the RFC complex. |
==About this Structure== | ==About this Structure== | ||
| - | 2CHV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http:// | + | 2CHV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CHV OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Cook, N.]] | [[Category: Cook, N.]] | ||
| - | [[Category: Hall, D | + | [[Category: Hall, D R.]] |
[[Category: Seybert, A.]] | [[Category: Seybert, A.]] | ||
| - | [[Category: Singleton, M | + | [[Category: Singleton, M R.]] |
| - | [[Category: Wigley, D | + | [[Category: Wigley, D B.]] |
[[Category: aaa+ atpase]] | [[Category: aaa+ atpase]] | ||
[[Category: atp-binding]] | [[Category: atp-binding]] | ||
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[[Category: nucleotide-binding]] | [[Category: nucleotide-binding]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:48:46 2008'' |
Revision as of 14:48, 21 February 2008
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REPLICATION FACTOR C ADPNP COMPLEX
Overview
We have investigated the communication between subunits in replication factor C (RFC) from Archaeoglobus fulgidus. Mutation of the proposed arginine finger in the small subunits results in a complex that can still bind ATP but has impaired clamp-loading activity, a process that normally only requires binding of nucleotide. The small subunit alone forms a hexameric ring that is six-fold symmetric in the absence of ATP. However, this symmetry is broken when the nucleotide is bound to the complex. A conformational change associated with nucleotide binding may relate to the opening of PCNA rings by RFC during the loading reaction. The structures also reveal the importance of the N-terminal helix of each subunit at the ATP-binding site. Analysis of mutant protein complexes containing subunits lacking this N-terminal helix reveals key distinct regulatory roles during clamp loading that are different for the large and small subunits in the RFC complex.
About this Structure
2CHV is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.
Reference
Communication between subunits within an archaeal clamp-loader complex., Seybert A, Singleton MR, Cook N, Hall DR, Wigley DB, EMBO J. 2006 May 17;25(10):2209-18. Epub 2006 Apr 20. PMID:16628222
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