2kxq

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[[Image:2kxq.png|left|200px]]
 
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{{STRUCTURE_2kxq| PDB=2kxq | SCENE= }}
{{STRUCTURE_2kxq| PDB=2kxq | SCENE= }}
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===Solution Structure of Smurf2 WW2 and WW3 bound to Smad7 PY motif containing peptide===
===Solution Structure of Smurf2 WW2 and WW3 bound to Smad7 PY motif containing peptide===
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{{ABSTRACT_PUBMED_20937913}}
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{{ABSTRACT_PUBMED_20937913}}
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==Function==
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[[http://www.uniprot.org/uniprot/SMUF2_HUMAN SMUF2_HUMAN]] E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.<ref>PMID:11389444</ref><ref>PMID:12717440</ref>
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
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<ref group="xtra">PMID:020937913</ref><references group="xtra"/>
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<ref group="xtra">PMID:020937913</ref><references group="xtra"/><references/>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Chong, A.]]
[[Category: Chong, A.]]

Revision as of 20:03, 24 March 2013

Template:STRUCTURE 2kxq

Contents

Solution Structure of Smurf2 WW2 and WW3 bound to Smad7 PY motif containing peptide

Template:ABSTRACT PUBMED 20937913

Function

[SMUF2_HUMAN] E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.[1][2]

About this Structure

2kxq is a 2 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

  • Chong PA, Lin H, Wrana JL, Forman-Kay JD. Coupling of tandem Smad ubiquitination regulatory factor (Smurf) WW domains modulates target specificity. Proc Natl Acad Sci U S A. 2010 Oct 26;107(43):18404-9. Epub 2010 Oct 11. PMID:20937913 doi:10.1073/pnas.1003023107
  1. ↑ Bonni S, Wang HR, Causing CG, Kavsak P, Stroschein SL, Luo K, Wrana JL. TGF-beta induces assembly of a Smad2-Smurf2 ubiquitin ligase complex that targets SnoN for degradation. Nat Cell Biol. 2001 Jun;3(6):587-95. PMID:11389444 doi:10.1038/35078562
  2. ↑ Di Guglielmo GM, Le Roy C, Goodfellow AF, Wrana JL. Distinct endocytic pathways regulate TGF-beta receptor signalling and turnover. Nat Cell Biol. 2003 May;5(5):410-21. PMID:12717440 doi:10.1038/ncb975

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